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Apoptosis progression studied using parallel dielectrophoresis electrophysiological analysis and flow cytometry.

Mulhall, HJ, Cardnell, A, Hoettges, KF, Labeed, FH and Hughes, MP (2015) Apoptosis progression studied using parallel dielectrophoresis electrophysiological analysis and flow cytometry. Integr Biol (Camb).

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Abstract

Apoptosis is characterised by many cellular events, but the standard Annexin-V assay identifies two; the transfer of the phospholipid phosphatidylserine (PS) from inner to outer leaflets of the plasma membrane, acting as an "eat me" signal to macrophages, and the permeabilisation of the plasma membrane. In this paper we compare the results from the Annexin-V assay with electrophysiology data obtained in parallel using dielectrophoresis, which highlights two changes in cell electrophysiology; a change in cytoplasmic conductivity which correlates with PS expression, and a membrane conductance spike that correlates with permeabilisation. Combining results from both methods shows a strong inverse relationship between conductivity and PS externalisation. One mechanism which may explain this correlation is related to intracellular Ca(2+), which is known to increase early in apoptosis. PS expression occurs when enzymes called scramblases swap external and internal phospholipids, and which are usually activated by Ca(2+), whilst the change in cytoplasmic conductivity may be due to K(+) efflux from intermediate conductance (IK) ion channels that are also activated by Ca(2+).

Item Type: Article
Authors :
AuthorsEmailORCID
Mulhall, HJUNSPECIFIEDUNSPECIFIED
Cardnell, AUNSPECIFIEDUNSPECIFIED
Hoettges, KFUNSPECIFIEDUNSPECIFIED
Labeed, FHUNSPECIFIEDUNSPECIFIED
Hughes, MPUNSPECIFIEDUNSPECIFIED
Date : 3 August 2015
Identification Number : https://doi.org/10.1039/c5ib00109a
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 28 Mar 2017 10:55
Last Modified : 28 Mar 2017 10:55
URI: http://epubs.surrey.ac.uk/id/eprint/808462

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