University of Surrey

Test tubes in the lab Research in the ATI Dance Research

A genetic analysis of Trichuris trichiura and Trichuris suis from Ecuador.

Meekums, H, Hawash, MB, Sparks, AM, Oviedo, Y, Sandoval, C, Chico, ME, Stothard, JR, Cooper, PJ, Nejsum, P and Betson, M (2015) A genetic analysis of Trichuris trichiura and Trichuris suis from Ecuador. Parasit Vectors, 8.

[img]
Preview
Text
A genetic analysis of Trichuris trichiura and Trichuris suis from Ecuador..pdf - ["content_typename_Published version (Publisher's proof or final PDF)" not defined]
Available under License : See the attached licence file.

Download (504kB) | Preview
[img]
Preview
PDF (licence)
SRI_deposit_agreement.pdf
Available under License : See the attached licence file.

Download (33kB) | Preview

Abstract

BACKGROUND: Since the nematodes Trichuris trichiura and T. suis are morphologically indistinguishable, genetic analysis is required to assess epidemiological cross-over between people and pigs. This study aimed to clarify the transmission biology of trichuriasis in Ecuador. FINDINGS: Adult Trichuris worms were collected during a parasitological survey of 132 people and 46 pigs in Esmeraldas Province, Ecuador. Morphometric analysis of 49 pig worms and 64 human worms revealed significant variation. In discriminant analysis morphometric characteristics correctly classified male worms according to host species. In PCR-RFLP analysis of the ribosomal Internal Transcribed Spacer (ITS-2) and 18S DNA (59 pig worms and 82 human worms), nearly all Trichuris exhibited expected restriction patterns. However, two pig-derived worms showed a "heterozygous-type" ITS-2 pattern, with one also having a "heterozygous-type" 18S pattern. Phylogenetic analysis of the mitochondrial large ribosomal subunit partitioned worms by host species. Notably, some Ecuadorian T. suis clustered with porcine Trichuris from USA and Denmark and some with Chinese T. suis. CONCLUSION: This is the first study in Latin America to genetically analyse Trichuris parasites. Although T. trichiura does not appear to be zoonotic in Ecuador, there is evidence of genetic exchange between T. trichiura and T. suis warranting more detailed genetic sampling.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences
Authors :
AuthorsEmailORCID
Meekums, HUNSPECIFIEDUNSPECIFIED
Hawash, MBUNSPECIFIEDUNSPECIFIED
Sparks, AMUNSPECIFIEDUNSPECIFIED
Oviedo, YUNSPECIFIEDUNSPECIFIED
Sandoval, CUNSPECIFIEDUNSPECIFIED
Chico, MEUNSPECIFIEDUNSPECIFIED
Stothard, JRUNSPECIFIEDUNSPECIFIED
Cooper, PJUNSPECIFIEDUNSPECIFIED
Nejsum, PUNSPECIFIEDUNSPECIFIED
Betson, MUNSPECIFIEDUNSPECIFIED
Date : January 2015
Identification Number : 10.1186/s13071-015-0782-9
Related URLs :
Additional Information : © 2015 Meekums et al.; licensee BioMed Central. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Depositing User : Symplectic Elements
Date Deposited : 11 Aug 2015 14:13
Last Modified : 11 Aug 2015 14:13
URI: http://epubs.surrey.ac.uk/id/eprint/808137

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800