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Differential effects of basolateral and apical iron supply on iron transport in Caco-2 cells

Eady, JJ, Wormstone, YM, Heaton, SJ, Hilhorst, B and Elliott, RM (2015) Differential effects of basolateral and apical iron supply on iron transport in Caco-2 cells Genes and Nutrition, 10 (3), ARTN 1.

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Iron homeostasis in the human body is maintained primarily through regulation of iron absorption in the duodenum. The liver peptide hepcidin plays a central role in this regulation. Additionally, expression and functional control of certain components of the cellular iron transport machinery can be influenced directly by the iron status of enterocytes. The significance of this modulation, relative to the effects of hepcidin, and the comparative effects of iron obtained directly from the diet and/or via the bloodstream are not clear. The studies described here were performed using Caco-2 cell monolayers as a model of intestinal epithelium, to compare the effects of iron supplied in physiologically relevant forms to either the apical or basolateral surfaces of the cells. Both sources of iron provoked increased cellular ferritin content, indicating iron uptake from both sides of the cells. Supply of basolateral transferrin-bound iron did not affect subsequent iron transport across the apical surface, but reduced iron transport across the basolateral membrane. In contrast, the apical iron supply led to subsequent reduction in iron transport across the apical cell membrane without altering iron export across the basolateral membrane. The apical and basolateral iron supplies also elicited distinct effects on the expression and subcellular distribution of iron transporters. These data suggest that, in addition to the effects of cellular iron status on the expression of iron transporter genes, different modes and direction of iron supply to enterocytes can elicit distinct functional effects on iron transport.

Item Type: Article
Subjects : Nutrition
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine > Department of Nutritional Sciences
Authors :
Date : 1 May 2015
Identification Number : 10.1007/s12263-015-0463-5
Copyright Disclaimer : The final publication is available at Springer via
Uncontrolled Keywords : Science & Technology, Life Sciences & Biomedicine, Genetics & Heredity, Nutrition & Dietetics, Iron uptake, Iron transport, Iron metabolism, Iron homeostasis, Gene expression, Intestinal epithelium, INTESTINAL EPITHELIAL-CELLS, MESSENGER-RNA EXPRESSION, METAL-ION TRANSPORTER, SERUM-FREE MEDIUM, GENE-EXPRESSION, DIETARY IRON, REGULATORY PROTEINS, NONHEME IRON, ABSORPTION, HEPCIDIN
Related URLs :
Additional Information : The original publication is available at
Depositing User : Symplectic Elements
Date Deposited : 12 May 2015 10:20
Last Modified : 22 Apr 2016 01:08

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