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Expression of PGP 9.5 by enteric neurons in horses and donkeys with and without intestinal disease

Hudson, NPH, Pearson, GT, Mayhew, IG, Proudman, CJ, Burden, FA and Fintl, C (2014) Expression of PGP 9.5 by enteric neurons in horses and donkeys with and without intestinal disease Journal of Comparative Pathology, 150 (2-3). pp. 225-233.

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Abstract

Intestinal motility disorders are an important problem in horses and donkeys and this study was carried out in order to evaluate the enteric neurons in animals with and without intestinal disease. Surplus intestinal tissue samples were collected from 28 horses undergoing exploratory laparotomy for colic. In addition, surplus intestinal samples from 17 control horses were collected immediately following humane destruction for clinical conditions not relating to the intestinal tract. Similar samples were also collected during routine post-mortem examinations from 12 aged donkeys; six animals were humanely destroyed for conditions related to the intestinal tract, while the remaining six were humanely destroyed for other reasons including dental and orthopaedic diseases. Tissue samples were fixed in formalin and immunohistochemical labelling was performed targeting the enteric neurons using a polyclonal antibody specific for the neuronal marker PGP 9.5. The distribution and density of neuronal networks were assessed qualitatively and semiquantitatively. There was strong PGP 9.5 expression in both the horse and donkey samples and labelling was detected throughout the tissue sections. In both species, PGP 9.5-immunoreactive nerve fibres were detected in all layers of the intestinal tract, both in large and small intestinal samples. Networks of enteric neurons were present in the donkey with a similar distribution to that seen in the horse. There was no demonstrable difference in enteric neuronal density and distribution in the groups of animals with intestinal disease compared with those without, apart from two (out of 28) horses with intestinal disease that showed a marked reduction in PGP 9.5 immunoreactivity. Apart from these two animals, this total cohort analysis differs from some previously observed findings in horses with intestinal disease and may therefore reflect the different pathophysiological processes occurring in varying intestinal conditions resulting in colic both in the donkey and the horse. © 2013 Elsevier Ltd.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Veterinary Medicine
Authors :
AuthorsEmailORCID
Hudson, NPHUNSPECIFIEDUNSPECIFIED
Pearson, GTUNSPECIFIEDUNSPECIFIED
Mayhew, IGUNSPECIFIEDUNSPECIFIED
Proudman, CJUNSPECIFIEDUNSPECIFIED
Burden, FAUNSPECIFIEDUNSPECIFIED
Fintl, CUNSPECIFIEDUNSPECIFIED
Date : February 2014
Identification Number : 10.1016/j.jcpa.2013.11.203
Additional Information : NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Comparative Pathology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Comparative Pathology, 150(2-3),February 2014, DOI 10.1016/j.jcpa.2013.11.203.
Depositing User : Symplectic Elements
Date Deposited : 04 Feb 2015 12:18
Last Modified : 04 Feb 2015 12:18
URI: http://epubs.surrey.ac.uk/id/eprint/807117

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