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Limited interlaboratory comparison of Schmallenberg virus antibody detection in serum samples.

van der Poel, WH, Cay, B, Zientara, S, Steinbach, F, Valarcher, JF, Bøtner, A, Mars, MH, Hakze-van der Honing, R, Schirrmeier, H and Beer, M (2014) Limited interlaboratory comparison of Schmallenberg virus antibody detection in serum samples. Vet Rec, 174 (15).

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Abstract

Eight veterinary institutes in seven different countries in Europe participated in a limited interlaboratory comparison trial to evaluate laboratory performances of Schmallenberg virus (SBV) antibody detection in serum. Seven different sheep sera and three different cattle sera were circulated, and all participating institutes were asked to test these sera using SBV antibody detection assay(s) in place in their laboratories. All laboratories within the trial performed a virus neutralisation test (VNT) as well as one or two ELISAs on all samples, and swiftly detected SBV antibodies using these assays. VNT was more sensitive in detecting SBV antibodies than several of the used ELISA assays. Based on the test results, one cattle and one sheep SBV antibody-positive serum were selected to serve as reference sera, which now can be supplied to other laboratories on request.

Item Type: Article
Authors :
AuthorsEmailORCID
van der Poel, WHUNSPECIFIEDUNSPECIFIED
Cay, BUNSPECIFIEDUNSPECIFIED
Zientara, SUNSPECIFIEDUNSPECIFIED
Steinbach, FUNSPECIFIEDUNSPECIFIED
Valarcher, JFUNSPECIFIEDUNSPECIFIED
Bøtner, AUNSPECIFIEDUNSPECIFIED
Mars, MHUNSPECIFIEDUNSPECIFIED
Hakze-van der Honing, RUNSPECIFIEDUNSPECIFIED
Schirrmeier, HUNSPECIFIEDUNSPECIFIED
Beer, MUNSPECIFIEDUNSPECIFIED
Date : 12 April 2014
Identification Number : https://doi.org/10.1136/vr.102180
Uncontrolled Keywords : Animals, Antibodies, Viral, Bunyaviridae Infections, Cattle, Cattle Diseases, Enzyme-Linked Immunosorbent Assay, Europe, Neutralization Tests, Orthobunyavirus, Sensitivity and Specificity, Sheep, Sheep Diseases
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 28 Mar 2017 10:52
Last Modified : 28 Mar 2017 10:52
URI: http://epubs.surrey.ac.uk/id/eprint/807083

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