University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Detection of Schmallenberg virus serum neutralising antibodies.

Mansfield, KL, La Rocca, SA, Khatri, M, Johnson, N, Steinbach, F and Fooks, AR (2013) Detection of Schmallenberg virus serum neutralising antibodies. J Virol Methods, 188 (1-2). pp. 139-144.

[img] Text
56 Mansfield JVM 2013.pdf - ["content_typename_UNSPECIFIED" not defined]
Restricted to Repository staff only
Available under License : See the attached licence file.

Download (402kB)
[img] PDF (licence)
SRI_deposit_agreement.pdf
Restricted to Repository staff only
Available under License : See the attached licence file.

Download (33kB)

Abstract

Schmallenberg virus (SBV) emerged in continental Europe in late 2011, and further work is required to assess the prevalence of SBV throughout Europe. Since its detection in Germany, SBV has now been detected in other European countries, including the United Kingdom. Infection with SBV can cause mild clinical signs in ruminants, including diarrhoea and reduced milk yield. However, the virus can have a devastating effect on the developing foetus leading to malformation in newborn offspring. This is a feature shared by other members of the Simbu group of orthobunyaviruses. Since disease in adult animals can be inapparent, serology offers the best method for monitoring for the presence of SBV and assisting in livestock management. This protocol describes a method for initial titration of SBV on African Green Monkey kidney (Vero) cells, and a plaque reduction neutralisation test (PRNT) for the detection of neutralising antibodies against SBV in cattle and sheep sera. This assay can be used to screen ruminant sera in order to confirm exposure to the virus, and the results obtained are comparable to a recently developed commercial enzyme linked immunosorbent assay (ELISA). Thus, these two assays constitute an effective diagnostic tool-box for providing confirmation of exposure to SBV.

Item Type: Article
Authors :
AuthorsEmailORCID
Mansfield, KLUNSPECIFIEDUNSPECIFIED
La Rocca, SAUNSPECIFIEDUNSPECIFIED
Khatri, MUNSPECIFIEDUNSPECIFIED
Johnson, NUNSPECIFIEDUNSPECIFIED
Steinbach, FUNSPECIFIEDUNSPECIFIED
Fooks, ARUNSPECIFIEDUNSPECIFIED
Date : March 2013
Identification Number : https://doi.org/10.1016/j.jviromet.2012.11.031
Uncontrolled Keywords : Animals, Antibodies, Neutralizing, Antibodies, Viral, Bunyaviridae Infections, Cattle, Cattle Diseases, Cercopithecus aethiops, Neutralization Tests, Orthobunyavirus, Serologic Tests, Sheep, Sheep Diseases, Vero Cells, Veterinary Medicine, Viral Plaque Assay
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 28 Mar 2017 10:51
Last Modified : 28 Mar 2017 10:51
URI: http://epubs.surrey.ac.uk/id/eprint/806878

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800