University of Surrey

Test tubes in the lab Research in the ATI Dance Research

CD4+CD25+ T cells expressing FoxP3 in Icelandic horses affected with insect bite hypersensitivity.

Hamza, E, Steinbach, F and Marti, E (2012) CD4+CD25+ T cells expressing FoxP3 in Icelandic horses affected with insect bite hypersensitivity. Vet Immunol Immunopathol, 148 (1-2). pp. 139-144.

[img] Text
53 Hamza VII 2012.pdf - ["content_typename_UNSPECIFIED" not defined]
Restricted to Repository staff only
Available under License : See the attached licence file.

Download (632kB)
[img] PDF (licence)
SRI_deposit_agreement.pdf
Restricted to Repository staff only
Available under License : See the attached licence file.

Download (33kB)

Abstract

Insect bite hypersensitivity (IBH) is an IgE-mediated dermatitis caused by bites of midges from the genus Culicoides. We have shown previously that peripheral blood mononuclear cells (PBMC) from IBH-affected horses produce higher levels of IL-4 and lower levels of IL-10 and TGF-β1 than those from healthy horses, suggesting that IBH is associated with a reduced regulatory immune response. FoxP3 is a crucial marker of regulatory T cells (Tregs). Here we have determined the proportion of CD4(+)CD25(+)FoxP3(+) T cells by flow cytometry in PBMC directly after isolation or after stimulation with Culicoides extract or a control antigen (Tetanus Toxoid). There were no differences between healthy and IBH horses either in the proportion of FoxP3(+)CD4(+)CD25(+) cells in freshly isolated PBMC or in the following stimulation with Tetanus Toxoid. However, upon stimulation of PBMC with the allergen, expression of FoxP3 by CD4(+)CD25(+high) and CD4(+)CD25(+dim) cells was significantly higher in healthy than in IBH horses. Addition of recombinant IL-4 to PBMC from healthy horses stimulated with the allergen significantly decreased the proportion of FoxP3 expressing cells within CD4(+)CD25(+high). These results suggest that IBH is associated with a decreased number of allergen-induced Tregs. This could be a consequence of the increased IL-4 production by PBMC of IBH-affected horses.

Item Type: Article
Authors :
AuthorsEmailORCID
Hamza, EUNSPECIFIEDUNSPECIFIED
Steinbach, FUNSPECIFIEDUNSPECIFIED
Marti, EUNSPECIFIEDUNSPECIFIED
Date : 15 July 2012
Identification Number : https://doi.org/10.1016/j.vetimm.2011.05.033
Uncontrolled Keywords : Animals, Cells, Cultured, Ceratopogonidae, Flow Cytometry, Forkhead Transcription Factors, Horse Diseases, Horses, Hypersensitivity, Insect Bites and Stings, Leukocytes, Mononuclear, T-Lymphocytes, Regulatory
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 28 Mar 2017 10:51
Last Modified : 28 Mar 2017 10:51
URI: http://epubs.surrey.ac.uk/id/eprint/806872

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800