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Classical swine fever virus infection protects aortic endothelial cells from pIpC-mediated apoptosis.

Johns, HL, Bensaude, E, La Rocca, SA, Seago, J, Charleston, B, Steinbach, F, Drew, TW, Crooke, H and Everett, H (2010) Classical swine fever virus infection protects aortic endothelial cells from pIpC-mediated apoptosis. J Gen Virol, 91 (Pt 4). pp. 1038-1046.

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Abstract

Classical swine fever virus (CSFV) causes severe disease in pigs associated with leukopenia, haemorrhage and fever. We show that CSFV infection protects endothelial cells from apoptosis induced by the dsRNA mimic, pIpC, but not from other apoptotic stimuli, FasL or staurosporine. CSFV infection inhibits pIpC-induced caspase activation, mitochondrial membrane potential loss and cytochrome c release as well as the pro-apoptotic effects of truncated Bid (tBid) overexpression. The CSFV proteins N(pro) and E(rns) both contribute to CSFV inhibition of apoptosis. We conclude that CSFV infection can inhibit apoptotic signalling at multiple levels, including at the caspase-8 and the mitochondrial checkpoints. By supporting viral replication, endothelial cells may promote CSFV pathogenesis.

Item Type: Article
Authors :
NameEmailORCID
Johns, HLUNSPECIFIEDUNSPECIFIED
Bensaude, EUNSPECIFIEDUNSPECIFIED
La Rocca, SAUNSPECIFIEDUNSPECIFIED
Seago, JUNSPECIFIEDUNSPECIFIED
Charleston, BUNSPECIFIEDUNSPECIFIED
Steinbach, FUNSPECIFIEDUNSPECIFIED
Drew, TWUNSPECIFIEDUNSPECIFIED
Crooke, HUNSPECIFIEDUNSPECIFIED
Everett, HUNSPECIFIEDUNSPECIFIED
Date : April 2010
Identification Number : 10.1099/vir.0.016576-0
Uncontrolled Keywords : Animals, Aorta, Apoptosis, BH3 Interacting Domain Death Agonist Protein, Caspases, Cells, Cultured, Classical swine fever virus, Cytochromes c, Endothelial Cells, Enzyme Activation, RNA, Double-Stranded, Swine, Viral Envelope Proteins
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 28 Mar 2017 10:51
Last Modified : 31 Oct 2017 17:12
URI: http://epubs.surrey.ac.uk/id/eprint/806770

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