University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Hydrogen peroxide produced by glucose oxidase affects the performance of laccase cathodes in glucose/oxygen fuel cells: FAD-dependent glucose dehydrogenase as a replacement

Milton, RD, Slade, RCT, Giroud, F, Minteer, SD and Thumser, AE (2013) Hydrogen peroxide produced by glucose oxidase affects the performance of laccase cathodes in glucose/oxygen fuel cells: FAD-dependent glucose dehydrogenase as a replacement Physical Chemistry Chemical Physics, 15 (44). pp. 19371-19379.

[img] PDF (licence)
SRI_deposit_agreement.pdf
Restricted to Repository staff only
Available under License : See the attached licence file.

Download (33kB)
[img] Text
c3cp53351d.pdf - ["content_typename_Published version (Publisher's proof or final PDF)" not defined]
Restricted to Repository staff only
Available under License : See the attached licence file.

Download (1MB)

Abstract

Hydrogen peroxide production by glucose oxidase (GOx) and its negative effect on laccase performance have been studied. Simultaneously, FAD-dependent glucose dehydrogenase (FAD-GDH), an O-insensitive enzyme, has been evaluated as a substitute. Experiments focused on determining the effect of the side reaction of GOx between its natural electron acceptor O (consumed) and hydrogen peroxide (produced) in the electrolyte. Firstly, oxygen consumption was investigated by both GOx and FAD-GDH in the presence of substrate. Relatively high electrocatalytic currents were obtained with both enzymes. O consumption was observed with immobilized GOx only, whilst O concentration remained stable for the FAD-GDH. Dissolved oxygen depletion effects on laccase electrode performances were investigated with both an oxidizing and a reducing electrode immersed in a single compartment. In the presence of glucose, dramatic decreases in cathodic currents were recorded when laccase electrodes were combined with a GOx-based electrode only. Furthermore, it appeared that the major loss of performance of the cathode was due to the increase of HO concentration in the bulk solution induced laccase inhibition. 24 h stability experiments suggest that the use of O-insensitive FAD-GDH as to obviate in situ peroxide production by GOx is effective. Open-circuit potentials of 0.66 ± 0.03 V and power densities of 122.2 ± 5.8 μW cm were observed for FAD-GDH/laccase biofuel cells. © 2013 the Owner Societies.

Item Type: Article
Authors :
AuthorsEmailORCID
Milton, RDUNSPECIFIEDUNSPECIFIED
Slade, RCTUNSPECIFIEDUNSPECIFIED
Giroud, FUNSPECIFIEDUNSPECIFIED
Minteer, SDUNSPECIFIEDUNSPECIFIED
Thumser, AEUNSPECIFIEDUNSPECIFIED
Date : 28 November 2013
Identification Number : https://doi.org/10.1039/c3cp53351d
Depositing User : Symplectic Elements
Date Deposited : 28 Mar 2017 13:12
Last Modified : 28 Mar 2017 13:12
URI: http://epubs.surrey.ac.uk/id/eprint/806182

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800