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Two forms of human double-stranded RNA-specific editase 1 (hRED1) generated by the insertion of an Alu cassette

Gerber, A, O'Connell, MA and Keller, W (1997) Two forms of human double-stranded RNA-specific editase 1 (hRED1) generated by the insertion of an Alu cassette RNA, 3 (5). pp. 453-463.

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Abstract

The double-stranded RNA-specific editase 1 (RED1/ADAR2) is implicated in the editing of precursor-mRNAs (pre-mRNA) encoding subunits of glutamate receptors (GluRs) in brain. Site-specific deamination of adenosine to inosine alters the codon at the Q/R site in GluR-B rendering the heteromeric receptor impermeable to Ca ions. We cloned human RED1 (hRED1/hADAR2) cDNAs from a brain cDNA library. The human enzyme is 95% identical to the rat homologue. We characterized two alternatively spliced forms that differed by the presence of an Alu-J cassette in the deaminase domain. For the long form containing the Alu cassette, we isolated cDNA clones with an alternative C- terminus and 3'-UTR. An 8.8-kb transcript of hRED1 is most abundant in brain and heart, and lower levels are detected in other tissues. In vitro editing assays with purified recombinant hRED1 containing or lacking the Alu-J cassette revealed that both forms of the protein have the same substrate specificity, but differ in their catalytic activity.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine > Department of Microbial and Cellular Sciences
Authors :
AuthorsEmailORCID
Gerber, AUNSPECIFIEDUNSPECIFIED
O'Connell, MAUNSPECIFIEDUNSPECIFIED
Keller, WUNSPECIFIEDUNSPECIFIED
Date : May 1997
Additional Information : Copyright 1997 The Authors. Published by Cold Spring Harbor Laboratory Press.
Depositing User : Symplectic Elements
Date Deposited : 09 Jul 2014 09:54
Last Modified : 13 Sep 2014 01:33
URI: http://epubs.surrey.ac.uk/id/eprint/805615

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