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Carbon-Nanotube-Based Materials for Protein Crystallization

Asanithi, P, Saridakis, E, Govada, L, Jurewicz, I, Brunner, EW, Ponnusamy, R, Cleaver, JAS, Dalton, AB, Chayen, NE and Sear, RP (2009) Carbon-Nanotube-Based Materials for Protein Crystallization ACS APPL MATER INTER, 1 (6). pp. 1203-1210.

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Abstract

We report on the first use OF carbon-nanotube-based films to produce crystals of proteins. The crystals nucleate on the surface of the film. The difficulty of crystallizing proteins is a major bottleneck in. the determination of the structure and function of biological molecules. The crystallization of two model proteins and two medically relevant proteins was studied. Quantitative data on the crystallization times of the model protein lyozyme are also presented. Two types of nanotube films, one made with the surfactant Triton X-100 (TX-100) and one with gelatin, were tested. Both induce nucleation of the crystal phase at supersaturations at which the protein solution would otherwise remain clear: however, the gelatin-based film induced nucleation down to much lower supersaturations for the two model proteins with which it was used. it appears that the interactions of gelatin with the protein molecules are particularly favorable to nucleation. Crystals of the C I domain of the human cardiac myosin-binding protein-C that diffracted to a resolution of 1.6 angstrom were obtained on the TX-100 film. This is far superior to the best crystals obtained using standard techniques, which only diffracted to 3.0 angstrom. Thus, both of our nanotube-based films are very promising candidates for future work on crystallizing difficult-to-crystallize target proteins.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences > Physics
Authors :
AuthorsEmailORCID
Asanithi, PUNSPECIFIEDUNSPECIFIED
Saridakis, EUNSPECIFIEDUNSPECIFIED
Govada, LUNSPECIFIEDUNSPECIFIED
Jurewicz, IUNSPECIFIEDUNSPECIFIED
Brunner, EWUNSPECIFIEDUNSPECIFIED
Ponnusamy, RUNSPECIFIEDUNSPECIFIED
Cleaver, JASUNSPECIFIEDUNSPECIFIED
Dalton, ABUNSPECIFIEDUNSPECIFIED
Chayen, NEUNSPECIFIEDUNSPECIFIED
Sear, RPUNSPECIFIEDUNSPECIFIED
Date : June 2009
Identification Number : 10.1021/am9000858
Uncontrolled Keywords : carbon nanotubes, gelatin, nucleation, protein crystallization, nucleants, pores, SUPERSATURATED LYSOZYME SOLUTIONS, NUCLEATION, CRYSTALS, SURFACES, FIBERS, FILMS, FORM
Additional Information : This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Materials and Interfaces, copyright © American Chemical Society after peer review and technical editing by the publisher.To access the final edited and published work see http://dx.doi.org/10.1021/am9000858.
Depositing User : Symplectic Elements
Date Deposited : 01 Apr 2014 08:52
Last Modified : 09 Jun 2014 13:56
URI: http://epubs.surrey.ac.uk/id/eprint/805217

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