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Betacam: A commercial approach to β- Autoradiography

Cabello, J, Holland, A, Holland, K, Bailey, A, Kitchen, I and Wells, K (2009) Betacam: A commercial approach to β- Autoradiography In: Medical Imaging 2009: Physics of Medical Imaging, 2009-02-09 - 2009-02-12, Lake Buena Bista.

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Autoradiography is a well established imaging modality in Biology and Medicine. This aims to measure the location and concentration of labelled molecules within thin tissue sections. The brain is the most anatomically complex organ and identification of neuroanatomical structures is still a challenge particularly when small animals are used for pre-clinical trials. High spatial resolution and high sensitivity are therefore necessary. This work shows the performance and ability of a prototype commercial system, based on a Charged-Couple Device (CCD), to accurately obtain detailed functional information in brain Autoradiography. The sample is placed in contact with the detector enabling direct detection of β- particles in silicon, and the system is run in a range of quasi-room temperatures (17-22 °C) under stable conditions by using a precision temperature controller. Direct detection of β- particles with low energy down to ~5 keV from 3[H] is possible using this room temperature approach. The CCD used in this work is an E2V CCD47-20 frame-transfer device which removes the image smear arising in conventional full-frame imaging devices. The temporal stability of the system has been analyzed by exposing a set of 14[C] calibrated microscales for different periods of time, and measuring the stability of the resultant sensitivity and background noise. The thermal performance of the system has also been analyzed in order to demonstrate its capability of working in other life science applications, where higher working temperatures are required. Once the performance of the system was studied, a set of experiments with biological samples, labelled with typical β- radioisotopes, such as 3[H], has been carried out to demonstrate its application in life sciences.

Item Type: Conference or Workshop Item (Conference Paper)
Divisions : Faculty of Engineering and Physical Sciences > Electronic Engineering > Centre for Vision Speech and Signal Processing
Authors :
Date : 9 February 2009
Identification Number : 10.1117/12.811326
Additional Information : Copyright 2009 Proc. SPIE 7258, Medical Imaging 2009: Physics of Medical Imaging. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. J. Cabello, A. Holland, K. Holland, A. Bailey, I. Kitchen, K. Wells, "Betacam: a commercial approach to β-autoradiography", Proc. SPIE 7258, Medical Imaging 2009: Physics of Medical Imaging, 72583P (2009); doi:10.1117/12.811326
Depositing User : Symplectic Elements
Date Deposited : 24 Jan 2014 12:11
Last Modified : 11 Feb 2014 12:01

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