University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Validity of triple- and dual-tracer techniques to estimate glucose appearance.

Haidar, A, Elleri, D, Allen, JM, Harris, J, Kumareswaran, K, Nodale, M, Acerini, CL, Wilinska, ME, Jackson, N, Umpleby, AM, Evans, ML, Dunger, DB and Hovorka, R (2012) Validity of triple- and dual-tracer techniques to estimate glucose appearance. Am J Physiol Endocrinol Metab, 302 (12). E1493-E1501.

Full text not available from this repository.

Abstract

The triple-tracer (TT) dilution technique has been proposed to be the gold standard method to measure postprandial glucose appearance. However, validation against an independent standard has been missing. We addressed this issue and also validated the simpler dual-tracer (DT) technique. Sixteen young subjects with type 1 diabetes (age 19.5 ± 3.8 yr, BMI 23.4 ± 1.5 kg/m(2), HbA(1c) 8.7 ± 1.7%, diabetes duration 9.0 ± 6.9 yr, total daily insulin 0.9 ± 0.2 U·kg(-1)·day(-1), mean ± SD) received a variable intravenous 20% dextrose infusion enriched with [U-(13)C]glucose over 8 h to achieve postprandial-resembling glucose excursions while intravenous insulin was administered to achieve postprandial-resembling levels of plasma insulin. Primed [6,6-(2)H(2)]glucose was infused in a manner that mimicked the expected endogenous glucose production and [U-(13)C; 1,2,3,4,5,6,6-(2)H(7)]glucose was infused in a manner that mimicked the expected glucose appearance from a standard meal. Plasma glucose enrichment was measured by gas chromatography-mass spectrometry. The intravenous dextrose infusion served as an independent standard and was reconstructed using the TT and DT techniques with the two-compartment Radziuk/Mari model and an advanced stochastic computational method. The difference between the infused and reconstructed dextrose profile was similar for the two methods (root mean square error 6.6 ± 1.9 vs. 8.0 ± 3.5 μmol·kg(-1)·min(-1), TT vs. DT, P = NS, paired t-test). The TT technique was more accurate in recovering the overall dextrose infusion (100 ± 9 and 92 ± 12%; P = 0.02). The root mean square error associated with the mean dextrose infusion profile was 2.5 and 3.3 μmol·kg(-1)·min(-1) for the TT and DT techniques, respectively. We conclude that the TT and DT techniques combined with the advanced computational method can measure accurately exogenous glucose appearance. The TT technique tends to outperform slightly the DT technique, but the latter benefits from reduced experimental and computational complexity.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine > Department of Nutritional Sciences
Authors :
AuthorsEmailORCID
Haidar, AUNSPECIFIEDUNSPECIFIED
Elleri, DUNSPECIFIEDUNSPECIFIED
Allen, JMUNSPECIFIEDUNSPECIFIED
Harris, JUNSPECIFIEDUNSPECIFIED
Kumareswaran, KUNSPECIFIEDUNSPECIFIED
Nodale, MUNSPECIFIEDUNSPECIFIED
Acerini, CLUNSPECIFIEDUNSPECIFIED
Wilinska, MEUNSPECIFIEDUNSPECIFIED
Jackson, NUNSPECIFIEDUNSPECIFIED
Umpleby, AMUNSPECIFIEDUNSPECIFIED
Evans, MLUNSPECIFIEDUNSPECIFIED
Dunger, DBUNSPECIFIEDUNSPECIFIED
Hovorka, RUNSPECIFIEDUNSPECIFIED
Date : June 2012
Identification Number : 10.1152/ajpendo.00581.2011
Additional Information : Article is open access at the American Journal of Physiology Website: http://ajpendo.physiology.org/content/302/12/E1493
Depositing User : Symplectic Elements
Date Deposited : 23 Oct 2013 11:45
Last Modified : 08 Nov 2013 12:31
URI: http://epubs.surrey.ac.uk/id/eprint/804294

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800