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Using a large area CMOS APS for direct chemiluminescence detection in Western Blotting Electrophoresis

Esposito, M, Newcombe, J, Anaxagoras, T, Allinson, NM and Wells, K (2012) Using a large area CMOS APS for direct chemiluminescence detection in Western Blotting Electrophoresis In: SPIE Medical Imaging Conference, 2012-02-04 - 2012-02-09, San Diego, USA.

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Western blotting electrophoretic sequencing is an analytical technique widely used in Functional Proteomics to detect, recognize and quantify specific labelled proteins in biological samples. A commonly used label for western blotting is Enhanced ChemiLuminescence (ECL) reagents based on fluorescent light emission of Luminol at 425nm. Film emulsion is the conventional detection medium, but is characterized by non-linear response and limited dynamic range. Several western blotting digital imaging systems have being developed, mainly based on the use of cooled Charge Coupled Devices (CCDs) and single avalanche diodes that address these issues. Even so these systems present key drawbacks, such as a low frame rate and require operation at low temperature. Direct optical detection using Complementary Metal Oxide Semiconductor (CMOS) Active Pixel Sensors (APS)could represent a suitable digital alternative for this application. In this paper the authors demonstrate the viability of direct chemiluminescent light detection in western blotting electrophoresis using a CMOS APS at room temperature. Furthermore, in recent years, improvements in fabrication techniques have made available reliable processes for very large imagers, which can be now scaled up to wafer size, allowing direct contact imaging of full size western blotting samples. We propose using a novel wafer scale APS (12.8 cm×13.2 cm), with an array architecture using two different pixel geometries that can deliver an inherently low noise and high dynamic range image at the same time representing a dramatic improvement with respect to the current western blotting imaging systems.

Item Type: Conference or Workshop Item (Conference Paper)
Divisions : Faculty of Engineering and Physical Sciences > Electronic Engineering
Authors :
Date : 5 April 2012
Identification Number : 10.1117/12.911615
Uncontrolled Keywords : western blotting, protein detection, proteomics, room temperature image sensor, CMOS, APS
Additional Information : Copyright 2012 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.
Depositing User : Symplectic Elements
Date Deposited : 27 Sep 2013 18:11
Last Modified : 27 Sep 2013 18:11

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