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Western Blotting Electrophoretic Sequencing: First Images with a Room Temperature CMOS Detector

Esposito, M, Newcombe, J and Wells, K Western Blotting Electrophoretic Sequencing: First Images with a Room Temperature CMOS Detector In: 2011 NSS/MIC Conference, 2011-10-23 - 2011-10-29, Valencia, Spain.

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Abstract

CMOS imaging technology can be applied to Chemiluminescence Western Blotting as a potential imaging alternative technology to using conventional film-emulsion. In this work the authors present a through investigation on the performance of CMOS Active Pixel Sensors for using in western blotting. Chemiluminescence labeling is a well established technique to detect proteins and presents several advantages compared with the fluorescence labeling. In fact it requires neither external illumination nor filtering optics and does not produce an inherently label-related background to correct. In this paper the first results of imaging a secondary antibody labeled with chemiluminescence reagents obtained with a CMOS sensor operating at room temperature are presented

Item Type: Conference or Workshop Item (Conference Paper)
Divisions : Faculty of Engineering and Physical Sciences > Electronic Engineering
Authors :
AuthorsEmailORCID
Esposito, MUNSPECIFIEDUNSPECIFIED
Newcombe, JUNSPECIFIEDUNSPECIFIED
Wells, KUNSPECIFIEDUNSPECIFIED
Identification Number : 10.1109/NSSMIC.2011.6152675
Contributors :
ContributionNameEmailORCID
PublisherIEEE, UNSPECIFIEDUNSPECIFIED
Uncontrolled Keywords : CMOS, APS, Room temperature image sensor, Electrophoresis, Western blot, Enhanced chemiluminescence
Additional Information : © 2011 IEEE. Personal use of this material is permitted. Permission from IEEE must be obtained for all other uses, in any current or future media, including reprinting/republishing this material for advertising or promotional purposes, creating new collective works, for resale or redistribution to servers or lists, or reuse of any copyrighted component of this work in other works.
Depositing User : Symplectic Elements
Date Deposited : 27 Sep 2013 18:00
Last Modified : 09 Jun 2014 13:11
URI: http://epubs.surrey.ac.uk/id/eprint/802299

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