University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Cytoplasm resistivity of Mammalian atrial myocardium determined by dielectrophoresis and impedance methods.

Fry, CH, Salvage, SC, Manazza, A, Dupont, E, Labeed, FH, Hughes, MP and Jabr, RI (2012) Cytoplasm resistivity of Mammalian atrial myocardium determined by dielectrophoresis and impedance methods. Biophys J, 103 (11). pp. 2287-2294.

[img] Text
RJabr-Biophysical J 2012.pdf
Restricted to Repository staff only
Available under License : See the attached licence file.

Download (593kB)
[img] Text (licence)
Restricted to Repository staff only

Download (33kB)


Many cardiac arrhythmias are caused by slowed conduction of action potentials, which in turn can be due to an abnormal increase of intracellular myocardial resistance. Intracellular resistivity is a linear sum of that offered by gap junctions between contiguous cells and the cytoplasm of the myocytes themselves. However, the relative contribution of the two components is unclear, especially in atrial myocardium, as there are no precise measurements of cytoplasmic resistivity, R(c). In this study, R(c) was measured in atrial tissue using several methods: a dielectrophoresis technique with isolated cells and impedance measurements with both isolated cells and multicellular preparations. All methods yielded similar values for R(c), with a mean of 138 ± 5 Ω·cm at 23°C, and a Q(10) value of 1.20. This value is about half that of total intracellular resistivity and thus will be a significant determinant of the actual value of action potential conduction velocity. The dielectrophoresis experiments demonstrated the importance of including divalent cations (Ca(2+) and Mg(2+)) in the suspension medium, as their omission reduced cell integrity by lowering membrane resistivity and increasing cytoplasm resistivity. Accurate measurement of R(c) is essential to develop quantitative computational models that determine the key factors contributing to the development of cardiac arrhythmias.

Item Type: Article
Authors :
Fry, CH
Salvage, SC
Manazza, A
Dupont, E
Labeed, FH
Hughes, MP
Jabr, RI
Date : 5 December 2012
DOI : 10.1016/j.bpj.2012.10.023
Depositing User : Symplectic Elements
Date Deposited : 28 Mar 2017 13:53
Last Modified : 31 Oct 2017 15:10

Actions (login required)

View Item View Item


Downloads per month over past year

Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800