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Concentration of various trace elements in the rat retina and their distribution in different structures.

Ugarte, M, Grime, GW, Lord, G, Geraki, K, Collingwood, JF, Finnegan, ME, Farnfield, H, Merchant, M, Bailey, MJ, Ward, NI , Foster, PJ, Bishop, PN and Osborne, NN (2012) Concentration of various trace elements in the rat retina and their distribution in different structures. Metallomics, 4 (12). pp. 1245-1254.

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Abstract

Inductively coupled plasma mass spectrometry (ICP-MS) was used to quantify the total amount of trace elements in retina from adult male Sprague-Dawley rats (n = 6). Concentration of trace elements within individual retinal areas in frozen sections of the fellow eye was established with the use of two methodologies: (1) particle-induced X-ray emission (PIXE) in combination with 3D depth profiling with Rutherford backscattering spectrometry (RBS) and (2) synchrotron X-ray fluorescence (SXRF) microscopy. The most abundant metal in the retina was zinc, followed by iron and copper. Nickel, manganese, chromium, cobalt, selenium and cadmium were present in very small amounts. The PIXE and SXRF analysis yielded a non-homogenous pattern distribution of metals in the retina. Relatively high levels of zinc were found in the inner part of the photoreceptor inner segments (RIS)/outer limiting membrane (OLM), inner nuclear layer and plexiform layers. Iron was found to accumulate in the retinal pigment epithelium/choroid layer and RIS/OLM. Copper in turn, was localised primarily in the RIS/OLM and plexiform layers. The trace elements iron, copper, and zinc exist in different amounts and locations in the rat retina.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences > Chemistry
Authors :
NameEmailORCID
Ugarte, MUNSPECIFIEDUNSPECIFIED
Grime, GWUNSPECIFIEDUNSPECIFIED
Lord, GUNSPECIFIEDUNSPECIFIED
Geraki, KUNSPECIFIEDUNSPECIFIED
Collingwood, JFUNSPECIFIEDUNSPECIFIED
Finnegan, MEUNSPECIFIEDUNSPECIFIED
Farnfield, HUNSPECIFIEDUNSPECIFIED
Merchant, MUNSPECIFIEDUNSPECIFIED
Bailey, MJUNSPECIFIEDUNSPECIFIED
Ward, NIUNSPECIFIEDUNSPECIFIED
Foster, PJUNSPECIFIEDUNSPECIFIED
Bishop, PNUNSPECIFIEDUNSPECIFIED
Osborne, NNUNSPECIFIEDUNSPECIFIED
Date : 21 December 2012
Identification Number : 10.1039/c2mt20157g
Depositing User : Symplectic Elements
Date Deposited : 28 Mar 2017 13:51
Last Modified : 31 Oct 2017 15:09
URI: http://epubs.surrey.ac.uk/id/eprint/795073

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