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Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool

Piluso, S, Cassell, HC, Gibbons, JL, Waller, TE, Plant, NJ, Miller, AF and Cavalli, G (2013) Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool Soft Matter, 9 (29). pp. 6752-6756.

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Abstract

The site-specific conjugation of DNA-binding protein (Tus) to self-assembling peptide FEFEFKFKK was demonstrated. Rheology studies and TEM of the corresponding hydrogels (including PNIPAAm-containing systems) showed no significant variation in properties and hydrogel morphology compared to FEFEFKFKK. Critically, we demonstrate that Tus is accessible within the gel network displaying DNA-binding properties.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences > Chemistry
Authors :
AuthorsEmailORCID
Piluso, SUNSPECIFIEDUNSPECIFIED
Cassell, HCUNSPECIFIEDUNSPECIFIED
Gibbons, JLUNSPECIFIEDUNSPECIFIED
Waller, TEUNSPECIFIEDUNSPECIFIED
Plant, NJUNSPECIFIEDUNSPECIFIED
Miller, AFUNSPECIFIEDUNSPECIFIED
Cavalli, GUNSPECIFIEDUNSPECIFIED
Date : 7 August 2013
Identification Number : 10.1039/c3sm00131h
Related URLs :
Additional Information : This Open Access Article is licensed under a Creative Commons Attribution 3.0 Unported Licence
Depositing User : Symplectic Elements
Date Deposited : 23 Jul 2013 11:25
Last Modified : 23 Sep 2013 20:13
URI: http://epubs.surrey.ac.uk/id/eprint/791727

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