Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool
Piluso, S, Cassell, HC, Gibbons, JL, Waller, TE, Plant, NJ, Miller, AF and Cavalli, G (2013) Site-specific, covalent incorporation of Tus, a DNA-binding protein, on ionic-complementary self-assembling peptide hydrogels using transpeptidase Sortase A as a conjugation tool Soft Matter, 9 (29). pp. 6752-6756.
c3sm00131h.pdf - Version of Record
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The site-specific conjugation of DNA-binding protein (Tus) to self-assembling peptide FEFEFKFKK was demonstrated. Rheology studies and TEM of the corresponding hydrogels (including PNIPAAm-containing systems) showed no significant variation in properties and hydrogel morphology compared to FEFEFKFKK. Critically, we demonstrate that Tus is accessible within the gel network displaying DNA-binding properties.
|Divisions :||Faculty of Engineering and Physical Sciences > Chemistry|
|Date :||7 August 2013|
|Identification Number :||https://doi.org/10.1039/c3sm00131h|
|Related URLs :|
|Additional Information :||This Open Access Article is licensed under a Creative Commons Attribution 3.0 Unported Licence|
|Depositing User :||Symplectic Elements|
|Date Deposited :||23 Jul 2013 11:25|
|Last Modified :||23 Sep 2013 20:13|
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