Optimising DNA binding to carbon nanotubes by non-covalent methods
Sanz, V, Borowiak, E, Lukanov, P, Galibert, AM, Flahaut, E, Coley, HM, Silva, SRP and McFadden, J (2011) Optimising DNA binding to carbon nanotubes by non-covalent methods Carbon, 49 (5). 1775 - 1781. ISSN 0008-6223
Manuscript Carbon 1.pdf - Accepted Version
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The use of carbon nanotubes as a gene delivery system has been extensively studied in recent years owing to its potential advantages over viral vectors. To achieve this goal, carbon nanotubes have to be functionalized to become compatible with aqueous media and to bind the genetic material. To establish the best conditions for plasmid DNA binding, we compare the dispersion properties of single-, double- and multi-walled carbon nanotubes (SWCNTs, DWCNTs and MWCNTs, respectively) functionalized with a variety of surfactants by non-covalent attachment. The DNA binding properties of the functionalized carbon nanotubes were studied and compared by electrophoresis. Furthermore, a bilayer functionalization method for DNA binding on SWCNTs was developed that utilized RNA-wrapping to solubilize the nanotubes and cationic polymers as a bridge between nanotubes and DNA.
|Additional Information:||NOTICE: this is the author’s version of a work that was accepted for publication in Carbon. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Carbon, 49(5), April 2011, DOI 10.1016/j.carbon.2010.12.064.|
|Divisions:||Faculty of Health and Medical Sciences > Microbial and Cellular Sciences|
|Depositing User:||Symplectic Elements|
|Date Deposited:||01 Dec 2011 13:46|
|Last Modified:||09 Jun 2014 13:24|
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