Protein detection using hydrogel-based molecularly imprinted polymers integrated with dual polarisation interferometry
Reddy, SM, Hawkins, DM, Phan, QT, Stevenson, D and Warriner, K (2013) Protein detection using hydrogel-based molecularly imprinted polymers integrated with dual polarisation interferometry Sensors and Actuators, B: Chemical, 176 (Januar). pp. 190-197.
A polyacrylamide-based molecularly imprinted polymer (MIP) was prepared for bovine haemoglobin (BHb). A 3 mg/ml solution of BHb was injected over a dual polarisation interferometer (DPI) sensor to form a physisorbed layer typically of 3.5 ± 0.5 nm thickness. Onto the pre-adsorbed protein layer, MIP and NIP (non-imprinted polymer) were separately injected to monitor the interaction of BHb MIP or NIP particles under different loading conditions with the pre-adsorbed protein layer. In the case of NIP flowing of the protein layer, there was negligible surface stripping of the pre-adsorbed protein. When a protein-eluted sample of MIP particles was flowed over a pre-adsorbed protein layer on the sensor chip, the sensor detected significant decreases in both layer thickness and mass, suggestive that protein was being selectively bound to MIP after being stripped-off from the sensor surface. We also integrated thin-film MIPS for BHb and BSA onto the DPI sensor surface and were able to show that whereas BHb bound selectively and strongly to the BHb MIP thin film (resulting in a sustained increase in thickness and mass), the BHb protein only demonstrated transient and reversible binding on the BSA MIP. MIPs were also tested after biofouling with plasma or serum at various dilutions. We found that serum at 1/100 dilution allowed the MIP to still function selectively. This is the first demonstration of MIPs being integrated with DPI in the development of synthetic receptor-based optical protein sensors. © 2012 Elsevier B.V. All rights reserved.
|Divisions :||Faculty of Engineering and Physical Sciences > Chemistry|
|Identification Number :||https://doi.org/10.1016/j.snb.2012.10.007|
|Additional Information :||NOTICE: this is the author’s version of a work that was accepted for publication in Sensors and Actuators, B: Chemical. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Sensors and Actuators, B: Chemical, 176, January 2013, DOI 10.1016/j.snb.2012.10.007.|
|Depositing User :||Symplectic Elements|
|Date Deposited :||04 Jul 2013 10:45|
|Last Modified :||25 Apr 2015 13:34|
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