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Expression of Engrailed-2 (EN2) protein in bladder cancer and its potential utility as a urinary diagnostic biomarker.

Morgan, R, Bryan, RT, Javed, S, Launchbury, F, Zeegers, MP, Cheng, KK, James, ND, Wallace, DM, Hurst, CD, Ward, DG, Knowles, MA and Pandha, H (2013) Expression of Engrailed-2 (EN2) protein in bladder cancer and its potential utility as a urinary diagnostic biomarker. Eur J Cancer.

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Abstract

Despite significant advances in our understanding of the molecular pathology of bladder cancer, it remains a significant health problem with high morbidity and mortality associated with muscle-invasive bladder cancer (stages T2+), and high costs associated with the surveillance of non-muscle-invasive bladder cancer (NMIBC, stages Ta/T1/Tis). Moreover, current diagnostic biomarkers are suboptimal and of poor utility for low grade disease and surveillance. In this study, we show that the Engrailed-2 (EN2) transcription factor is expressed in, and secreted by, bladder cancer cell lines and patient tumour specimens, justifying an evaluation of urinary EN2 as a diagnostic biomarker in bladder cancer using archived samples from an established biospecimen collection. In patients with NMIBC, urinary EN2 was detected in most cases with an overall sensitivity of 82% and specificity of 75%. The sensitivity for stage Ta and T1 tumours was 71% and 76%, respectively, and 94% for stage T2+ tumours. This compares favourably with existing markers. The sensitivity for tumour grades 1, 2 and 3 was 69%, 78% and 87%, respectively. Thus urinary EN2 has the potential to be a more sensitive and specific protein biomarker for NMIBC than currently available tests.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences
Authors :
AuthorsEmailORCID
Morgan, RUNSPECIFIEDUNSPECIFIED
Bryan, RTUNSPECIFIEDUNSPECIFIED
Javed, SUNSPECIFIEDUNSPECIFIED
Launchbury, FUNSPECIFIEDUNSPECIFIED
Zeegers, MPUNSPECIFIEDUNSPECIFIED
Cheng, KKUNSPECIFIEDUNSPECIFIED
James, NDUNSPECIFIEDUNSPECIFIED
Wallace, DMUNSPECIFIEDUNSPECIFIED
Hurst, CDUNSPECIFIEDUNSPECIFIED
Ward, DGUNSPECIFIEDUNSPECIFIED
Knowles, MAUNSPECIFIEDUNSPECIFIED
Pandha, HUNSPECIFIEDUNSPECIFIED
Date : 21 February 2013
Identification Number : 10.1016/j.ejca.2013.01.019
Related URLs :
Additional Information : NOTICE: this is the author’s version of a work that was accepted for publication in the European Journal of Cancer. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication.
Depositing User : Symplectic Elements
Date Deposited : 24 Apr 2013 08:18
Last Modified : 28 Nov 2014 15:28
URI: http://epubs.surrey.ac.uk/id/eprint/769595

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