University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Influence of surface modification on vitality and differentiation of Caco-2 cells

Piana, C, Güll, I, Wirth, M, Gabor, F, Gerbes, S, Gerdes, R, Mills, C and Samitier, J (2007) Influence of surface modification on vitality and differentiation of Caco-2 cells Differentiation, 75 (4). pp. 308-317.

[img] PDF
Gabor paper.pdf
Restricted to Repository staff only
Available under License : See the attached licence file.

Download (247kB)
[img] PDF (licence)
SRI_deposit_agreement.pdf
Restricted to Repository staff only

Download (33kB)

Abstract

It is widely accepted that the functional and morphological differentiation of cells is initiated and determined by the interaction of molecules of the extracellular matrix and adhesion molecules of the cell membrane. To assess the influence of the underlying matrix on the characteristics of cells, enterocyte-like Caco-2 cells were cultivated on substrates commonly used for cell culture as well as on glass coated with hydrophobic layers. Providing the same starting conditions for growth, the parameters investigated on preconfluent Caco-2 cells were the number of adhering cells, the proliferative activity and the degree of differentiation indicated by the expression of three brush border enzymes. Whereas tissue culture treated polystyrene elicited highest rates of adhesion, proliferation, and differentiation, even glass altered the pattern of brush border enzyme expression. The hydrophobic surfaces strongly decreased the adhesion and the proliferation but the surviving cells exhibited a pronounced higher degree of differentiation. Interestingly, each sub-type of hydrophobic matrix triggered a different pattern of brush border enzyme expression. Thus, the development of a certain phenotype of a cell can not only be triggered by certain components of the extracellular matrix but also by artificially prepared surface coatings of the underlying matrix. In the future it seems to be feasible that cells can be programmed by tailoring the surface of the underlying substrate. © 2007, International Society of Differentiation.

Item Type: Article
Authors :
AuthorsEmailORCID
Piana, CUNSPECIFIEDUNSPECIFIED
Güll, IUNSPECIFIEDUNSPECIFIED
Wirth, MUNSPECIFIEDUNSPECIFIED
Gabor, FUNSPECIFIEDUNSPECIFIED
Gerbes, SUNSPECIFIEDUNSPECIFIED
Gerdes, RUNSPECIFIEDUNSPECIFIED
Mills, CUNSPECIFIEDUNSPECIFIED
Samitier, JUNSPECIFIEDUNSPECIFIED
Date : April 2007
Identification Number : https://doi.org/10.1111/j.1432-0436.2006.00141.x
Depositing User : Symplectic Elements
Date Deposited : 28 Mar 2017 13:44
Last Modified : 28 Mar 2017 13:44
URI: http://epubs.surrey.ac.uk/id/eprint/755374

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800