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Widespread cytoplasmic mRNA transport in yeast: Identification of 22 bud-localized transcripts using DNA microarray analysis

Shepard, KA, Takizawa, PA, Vale, RD, Jambhekar, A, DeRisi, JL, Vale, RD, Gerber, AP, Brown, PO, Herschlag, D, Brown, PO and Takizawa, PA (2003) Widespread cytoplasmic mRNA transport in yeast: Identification of 22 bud-localized transcripts using DNA microarray analysis Proceedings of the National Academy of Sciences of the United States of America, 100 (20). pp. 11429-11434.

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Abstract

Cytoplasmic mRNA localization provides a means of generating cell asymmetry and segregating protein activity. Previous studies have identified two mRNAs that localize to the bud tips of the yeast Saccharomyces cerevisiae. To identify additional localized mRNAs, we immunoprecipitated the RNA transport components She2p, She3p, and Myo4p and performed DNA microarray analysis of their associated RNAs. A secondary screen, using a GFP-tagged RNA reporter assay, identified 22 mRNAs that are localized to bud tips. These messages encode a wide variety of proteins, including several involved in stress responses and cell wall maintenance. Many of these proteins are asymmetrically localized to buds. However, asymmetric localization also occurs in the absence of RNA transport, suggesting the existence of redundant protein localization mechanisms. In contrast to findings in metazoans, the untranslated regions are dispensable for mRNA localization in yeast. This study reveals an unanticipated widespread use of RNA transport in budding yeast.

Item Type: Article
Authors :
NameEmailORCID
Shepard, KAUNSPECIFIEDUNSPECIFIED
Takizawa, PAUNSPECIFIEDUNSPECIFIED
Vale, RDUNSPECIFIEDUNSPECIFIED
Jambhekar, AUNSPECIFIEDUNSPECIFIED
DeRisi, JLUNSPECIFIEDUNSPECIFIED
Vale, RDUNSPECIFIEDUNSPECIFIED
Gerber, APUNSPECIFIEDUNSPECIFIED
Brown, POUNSPECIFIEDUNSPECIFIED
Herschlag, DUNSPECIFIEDUNSPECIFIED
Brown, POUNSPECIFIEDUNSPECIFIED
Takizawa, PAUNSPECIFIEDUNSPECIFIED
Date : 30 September 2003
Identification Number : 10.1073/pnas.2033246100
Depositing User : Symplectic Elements
Date Deposited : 28 Mar 2017 14:12
Last Modified : 31 Oct 2017 14:53
URI: http://epubs.surrey.ac.uk/id/eprint/739706

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