University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Single Stem Cell Positioning on Polylysine and Fibronectin Microarrays

Ruiz, A, Zychowicz, M, Buzanska, L, Mehn, D, Mills, CA, Martinez, E, Coecke, S, Samitier, J, Colpo, P and Rossi, F (2009) Single Stem Cell Positioning on Polylysine and Fibronectin Microarrays MICRO AND NANOSYSTEMS, 1 (1). 50 - 56. ISSN 1876-4029

[img]
Preview
PDF
Micro and nanosystems Ana Ruiz.pdf
Available under License : See the attached licence file.

Download (979kB)
[img]
Preview
PDF (licence)
SRI_deposit_agreement.pdf

Download (33kB)

Abstract

Arrays of human umbilical cord blood-neural stem cells have been patterned in high density at single cell resolution. Pre-patterns of adhesive molecules, i.e. fibronectin and poly-L-lysine, have been produced on anti-adhesive poly (ethylene) oxide films deposited by plasma-enhanced chemical vapour deposition, which prevents cell adsorption. The structures consisted of adhesive squares and lines with 10μm lateral dimensions, which correspond approximately to the size of one cell nucleus, separated by 10μm anti-adhesive gap. The stem cells cultured on these platforms redistribute their cytoplasm on the permitted areas. Spherical cells were deposited on the square patterns in a single cell mode, while on the lines they spread longitudinally; the extent of elongation being dependent on the specific (fibronectin) or non-specific (poly-L-lysine) attachment biomolecule. The cell patterns were retained up to 12 days, which will be useful for recording statistical data of individual chronic responses to chemical, physical or physiologically relevant stimuli.

Item Type: Article
Additional Information: Copyright 2009 Bentham Science Publishers.
Uncontrolled Keywords: Micropatterning, single cell, cell adhesion, stem cell, polylysine, fibronectin
Related URLs:
Divisions: Faculty of Engineering and Physical Sciences > Electronic Engineering > Advanced Technology Institute > Nano-Electronics Centre
Depositing User: Symplectic Elements
Date Deposited: 03 Dec 2012 13:26
Last Modified: 23 Sep 2013 19:52
URI: http://epubs.surrey.ac.uk/id/eprint/738092

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800