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Dual wavelength multiple-angle light scattering system for cryptosporidium detection

Buaprathoom, S, Sweeney, SJ and Pedley, S (2012) Dual wavelength multiple-angle light scattering system for cryptosporidium detection Progress in Biomedical Optics and Imaging - Proceedings of SPIE, 8427. ISSN 1605-7422

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Abstract

A simple, dual wavelength, multiple-angle, light scattering system has been developed for detecting cryptosporidium suspended in water. Cryptosporidium is a coccidial protozoan parasite causing cryptosporidiosis; a diarrheal disease of varying severity. The parasite is transmitted by ingestion of contaminated water, particularly drinking-water, but also accidental ingestion of bathing-water, including swimming pools. It is therefore important to be able to detect these parasites quickly, so that remedial action can be taken to reduce the risk of infection. The proposed system combines multiple-angle scattering detection of a single and two wavelengths, to collect relative wavelength angle-resolved scattering phase functions from tested suspension, and multivariate data analysis techniques to obtain characterizing information of samples under investigation. The system was designed to be simple, portable and inexpensive. It employs two diode lasers (violet InGaN-based and red AlGaInP-based) as light sources and silicon photodiodes as detectors and optical components, all of which are readily available. The measured scattering patterns using the dual wavelength system showed that the relative wavelength angle-resolved scattering pattern of cryptosporidium oocysts was significantly different from other particles (e.g. polystyrene latex sphere, E.coli). The single wavelength set up was applied for cryptosporidium oocysts'size and relative refractive index measurement and differential measurement of the concentration of cryptosporidium oocysts suspended in water and mixed polystyrene latex sphere suspension. The measurement results showed good agreement with the control reference values. These results indicate that the proposed method could potentially be applied to online detection in a water quality control system. © 2012 SPIE.

Item Type: Article
Additional Information: Copyright 2012 Society of Photo-Optical Instrumentation Engineers. One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. S. Buaprathoom, S. Pedley, and S.J. Sweeney, "Dual wavelength multiple-angle light scattering system for cryptosporidium detection", Proc. SPIE 8427, 84272K (2012); doi:10.1117/12.921661
Divisions: Faculty of Engineering and Physical Sciences > Physics
Depositing User: Symplectic Elements
Date Deposited: 06 Nov 2012 09:52
Last Modified: 23 Sep 2013 19:44
URI: http://epubs.surrey.ac.uk/id/eprint/729747

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