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Design of double-walled carbon nanotubes for biomedical applications.

Neves, V, Heister, E, Costa, S, Tîlmaciu, C, Flahaut, E, Soula, B, Coley, HM, McFadden, J and Silva, SR (2012) Design of double-walled carbon nanotubes for biomedical applications. Nanotechnology, 23 (36). 365102 - ?. ISSN 0957-4484

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Abstract

Double-walled carbon nanotubes (DWNTs) prepared by catalytic chemical vapour deposition were functionalized in such a way that they were optimally designed as a nano-vector for the delivery of small interfering RNA (siRNA), which is of great interest for biomedical research and drug development. DWNTs were initially oxidized and coated with a polypeptide (Poly(Lys:Phe)), which was then conjugated to thiol-modified siRNA using a heterobifunctional cross-linker. The obtained oxDWNT-siRNA was characterized by Raman spectroscopy inside and outside a biological environment (mammalian cells). Uptake of the custom-designed nanotubes was not associated with detectable biochemical perturbations in cultured cells, but transfection of cells with DWNTs loaded with siRNA targeting the green fluorescent protein (GFP) gene, serving as a model system, as well as with therapeutic siRNA targeting the survivin gene, led to a significant gene silencing effect, and in the latter case a resulting apoptotic effect in cancer cells.

Item Type: Article
Additional Information: Copyright 2012 Institute of Physics. This is the author's accepted manuscript.
Divisions: Faculty of Engineering and Physical Sciences > Electronic Engineering > Advanced Technology Institute > Nano-Electronics Centre
Depositing User: Symplectic Elements
Date Deposited: 30 Oct 2012 09:35
Last Modified: 23 Sep 2013 19:42
URI: http://epubs.surrey.ac.uk/id/eprint/728016

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