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Metabolomic Profiling Can Differentiate Between Bactericidal Effects of Free and Polymer Bound Halogen

Ahmed, AESI, Wardell, JN, Thumser, AE, Avignone-Rossa, CA, Cavalli, G, Hay, JN and Bushell, ME (2011) Metabolomic Profiling Can Differentiate Between Bactericidal Effects of Free and Polymer Bound Halogen Journal of Applied Polymer Science, 119 (2). pp. 709-718.

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Abstract

The effects of two halogenated compounds (sodium hypochlorite and N-halamine polymers) on the Escherichia coli metabolome were investigated. Changes in the intracellular metabolite pools of bacterial cells treated with different formulations of these compounds were analysed using FTIR (Fourier Transform Infra Red) spectroscopy and LC-MS (Liquid Chromatography-Mass Spectroscopy). Principal component analysis was used to generate metabolic profiles of the intracellular metabolites to investigate the effect of sublethal concentrations on the metabolome of treated cells. The effect of treatment with sodium hypochlorite was quantitatively dependent on the exposure time. The resulting metabolic profiles supported our previous hypothesis that the mode of action of some halogenated compounds, such as N-halamine polymers, can be initiated by release of halogen ions into the aqueous environment, in addition to direct contact between the solid polymer material and the bacterial cells. Moreover, the metabolic profiles were able to differentiate between the effect of free and polymer-bound halogen. Our metabolomic approach was used for hypothesis generation to distinguish apparently different bactericidal effects of free and polymer-bound halogen.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine > Department of Microbial and Cellular Sciences
Faculty of Health and Medical Sciences > School of Biosciences and Medicine > Department of Biochemical Sciences
Faculty of Engineering and Physical Sciences > Chemistry
Authors :
AuthorsEmailORCID
Ahmed, AESIUNSPECIFIEDUNSPECIFIED
Wardell, JNUNSPECIFIEDUNSPECIFIED
Thumser, AEUNSPECIFIEDUNSPECIFIED
Avignone-Rossa, CAUNSPECIFIEDUNSPECIFIED
Cavalli, GUNSPECIFIEDUNSPECIFIED
Hay, JNUNSPECIFIEDUNSPECIFIED
Bushell, MEUNSPECIFIEDUNSPECIFIED
Date : 15 January 2011
Identification Number : 10.1002/app.32731
Uncontrolled Keywords : N-halamine, hypochlorite, metabolite, cells, LC-MS, PCA, N-HALAMINE, HYPOCHLOROUS ACID, BIOCIDAL POLYSTYRENE, SODIUM-HYPOCHLORITE, DEHYDROGENASE, METABOLITES, BEADS
Additional Information : This is the peer reviewed version of the following article: Ahmed, AESI, Wardell, JN, Thumser, AE, Avignone-Rossa, CA, Cavalli, G, Hay, JN and Bushell, ME (2011) Metabolomic Profiling Can Differentiate Between Bactericidal Effects of Free and Polymer Bound Halogen Journal of Applied Polymer Science, 119 (2). 709 - 718, which has been published in final form at http://dx.doi.org/10.1002/app.32731. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Depositing User : Symplectic Elements
Date Deposited : 06 Oct 2011 13:41
Last Modified : 02 Dec 2015 11:02
URI: http://epubs.surrey.ac.uk/id/eprint/7256

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