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Ascorbate enhances iron uptake into intestinal cells through formation of a FeCl3-ascorbate complex

Thumser, AE, Rashed, AA, Sharp, PA and Lodge, JK (2010) Ascorbate enhances iron uptake into intestinal cells through formation of a FeCl3-ascorbate complex Food Chemistry, 123 (2). 281 - 285. ISSN 0308-8146

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Abstract

It has been well documented that ascorbate enhances iron uptake, with a proposed mechanism based on reduction to the more absorbable ferrous form. We have performed a study on the effects of ascorbate on ferric iron uptake in the human epithelial Caco-2 cell-line. Ascorbate increased uptake in a concentration-dependent manner with a significant difference between iron uptake and reduction. Uptake kinetics are characteristic of a non-essential activator and the formation of an Fe3+–ascorbate complex. This investigation provides evidence that ascorbate enhances the apical uptake of ferric iron into Caco-2 cells through the formation of a Fe3+–ascorbate complex.

Item Type: Article
Uncontrolled Keywords: Enterocyte iron uptake, Ascorbate, Ferric iron, CACO-2 CELLS, NONHEME IRON, COLORIMETRIC ASSAY, EPITHELIAL-CELLS, EXPRESSION, TRANSPORT, ABSORPTION, DMT1, BIOAVAILABILITY, HUMANS
Divisions: Faculty of Health and Medical Sciences > Biochemistry and Physiology
Depositing User: Symplectic Elements
Date Deposited: 06 Oct 2011 11:36
Last Modified: 23 Sep 2013 18:45
URI: http://epubs.surrey.ac.uk/id/eprint/7253

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