Ascorbate enhances iron uptake into intestinal cells through formation of a FeCl3-ascorbate complex
Thumser, AE, Rashed, AA, Sharp, PA and Lodge, JK (2010) Ascorbate enhances iron uptake into intestinal cells through formation of a FeCl3-ascorbate complex Food Chemistry, 123 (2). 281 - 285.
Microsoft_Word_-_Thumser_et_al_Food_Chemistry_2010.pdf - Accepted version Manuscript
It has been well documented that ascorbate enhances iron uptake, with a proposed mechanism based on reduction to the more absorbable ferrous form. We have performed a study on the effects of ascorbate on ferric iron uptake in the human epithelial Caco-2 cell-line. Ascorbate increased uptake in a concentration-dependent manner with a significant difference between iron uptake and reduction. Uptake kinetics are characteristic of a non-essential activator and the formation of an Fe3+–ascorbate complex. This investigation provides evidence that ascorbate enhances the apical uptake of ferric iron into Caco-2 cells through the formation of a Fe3+–ascorbate complex.
|Divisions :||Faculty of Health and Medical Sciences > Biochemistry and Physiology|
|Date :||15 November 2010|
|Identification Number :||10.1016/j.foodchem.2010.04.031|
|Uncontrolled Keywords :||Enterocyte iron uptake, Ascorbate, Ferric iron, CACO-2 CELLS, NONHEME IRON, COLORIMETRIC ASSAY, EPITHELIAL-CELLS, EXPRESSION, TRANSPORT, ABSORPTION, DMT1, BIOAVAILABILITY, HUMANS|
|Depositing User :||Symplectic Elements|
|Date Deposited :||06 Oct 2011 11:36|
|Last Modified :||23 Sep 2013 18:45|
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