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Cellular localization, accumulation and trafficking of double-walled carbon nanotubes in human prostate cancer cells

Neves, V, Gerondopoulos, A, Heister, E, Tîlmaciu, C, Flahaut, E, Soula, B, Silva, SRP, McFadden, J and Coley, HM (2012) Cellular localization, accumulation and trafficking of double-walled carbon nanotubes in human prostate cancer cells Nano Research, 5 (4). 223 - 234. ISSN 1998-0124

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Abstract

Carbon nanotubes (CNTs) are at present being considered as potential nanovectors with the ability to deliver therapeutic cargoes into living cells. Previous studies established the ability of CNTs to enter cells and their therapeutic utility, but an appreciation of global intracellular trafficking associated with their cellular distribution has yet to be described. Despite the many aspects of the uptake mechanism of CNTs being studied, only a few studies have investigated internalization and fate of CNTs inside cells in detail. In the present study, intracellular localization and trafficking of RNA-wrapped, oxidized double-walled CNTs (oxDWNT-RNA) is presented. Fixed cells, previously exposed to oxDWNT-RNA, were subjected to immunocytochemical analysis using antibodies specific to proteins implicated in endocytosis; moreover cell compartment markers and pharmacological inhibitory conditions were also employed in this study. Our results revealed that an endocytic pathway is involved in the internalization of oxDWNT-RNA. The nanotubes were found in clathrin-coated vesicles, after which they appear to be sorted in early endosomes, followed by vesicular maturation, become located in lysosomes. Furthermore, we observed co-localization of oxDWNT-RNA with the small GTP-binding protein (Rab 11), involved in their recycling back to the plasma membrane via endosomes from the trans-golgi network.

Item Type: Article
Additional Information: The original publication is available at http://www.springerlink.com
Divisions: Faculty of Health and Medical Sciences > Microbial and Cellular Sciences
Faculty of Engineering and Physical Sciences > Electronic Engineering > Advanced Technology Institute > Nano-Electronics Centre
Faculty of Health and Medical Sciences > Biochemistry and Physiology
Depositing User: Symplectic Elements
Date Deposited: 29 Jun 2012 10:40
Last Modified: 23 Sep 2013 19:32
URI: http://epubs.surrey.ac.uk/id/eprint/711610

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