RIP-chip-SRM--a new combinatorial large-scale approach identifies a set of translationally regulated bantam/miR-58 targets in C. elegans.

Jovanovic, M, Reiter, L, Clark, A, Weiss, M, Picotti, P, Rehrauer, H, Frei, A, Neukomm, LJ, Kaufman, E, Wollscheid, B, Simard, MJ, Miska, EA, Aebersold, R, Gerber, AP and Hengartner, MO (2012) RIP-chip-SRM--a new combinatorial large-scale approach identifies a set of translationally regulated bantam/miR-58 targets in C. elegans. Genome Res.

	PDF Jovanovic_GenRes_2012.full.pdf Restricted to Repository staff only Available under License : See the attached licence file. Download (1MB)
	PDF (licence) SRI_deposit_agreement.pdf Restricted to Repository staff only Download (33kB)

Abstract

MicroRNAs (miRNAs) are small, noncoding RNAs that negatively regulate gene expression. As miRNAs are involved in a wide range of biological processes and diseases, much effort has been invested in identifying their mRNA targets. Here, we present a novel combinatorial approach, RIP-chip-SRM (RNA-binding protein immunopurification + microarray + targeted protein quantification via selected reaction monitoring), to identify de novo high-confidence miRNA targets in the nematode Caenorhabditis elegans. We used differential RIP-chip analysis of miRNA-induced silencing complexes from wild-type and miRNA mutant animals, followed by quantitative targeted proteomics via selected reaction monitoring to identify and validate mRNA targets of the C. elegans bantam homolog miR-58. Comparison of total mRNA and protein abundance changes in mir-58 mutant and wild-type animals indicated that the direct bantam/miR-58 targets identified here are mainly regulated at the level of protein abundance, not mRNA stability.

Item Type:	Article
Authors :	Authors Email ORCID Jovanovic, M UNSPECIFIED UNSPECIFIED Reiter, L UNSPECIFIED UNSPECIFIED Clark, A UNSPECIFIED UNSPECIFIED Weiss, M UNSPECIFIED UNSPECIFIED Picotti, P UNSPECIFIED UNSPECIFIED Rehrauer, H UNSPECIFIED UNSPECIFIED Frei, A UNSPECIFIED UNSPECIFIED Neukomm, LJ UNSPECIFIED UNSPECIFIED Kaufman, E UNSPECIFIED UNSPECIFIED Wollscheid, B UNSPECIFIED UNSPECIFIED Simard, MJ UNSPECIFIED UNSPECIFIED Miska, EA UNSPECIFIED UNSPECIFIED Aebersold, R UNSPECIFIED UNSPECIFIED Gerber, AP UNSPECIFIED UNSPECIFIED Hengartner, MO UNSPECIFIED UNSPECIFIED
Date :	19 April 2012
Identification Number :	https://doi.org/10.1101/gr.133330.111
Depositing User :	Symplectic Elements
Date Deposited :	28 Mar 2017 14:42
Last Modified :	28 Mar 2017 14:42
URI:	http://epubs.surrey.ac.uk/id/eprint/531404

Actions (login required)

View Item

Downloads