Molecular Biology II: Protein function
Plant, NJ (2012) Molecular Biology II: Protein function Surgery, 30 (4). pp. 169-173.
Protein Function (Surgery_12).pdf - Accepted version Manuscript
Available under License : See the attached licence file.
DNA may be viewed as the blueprint for the body, with mRNA/protein the component parts made from this blueprint. However, it is only when proteins are allowed to interact with each other and their surroundings that true biological complexity is achieved. Thus, while it is informative to study transcriptional control and mRNA transcript levels, it is equally important to assess the impact of the encoded proteins on the total cellular environment. For example, expression of a ligand-activated receptor such as the epidermal growth factor receptor is of no biological consequence if no EGF is present in the system. It is thus important to be able to study protein interactions and modification, enabling a comprehensive understanding of the biological mechanisms that underlie any particular phenotype. This article will outline the basic technologies to both visualise protein localisation and interaction between co-localised proteins. In addition, the manipulation of protein levels, both in vitro and in vivo, will be described, as this provides an important tool for the further examination of protein functionality within biological systems.
|Divisions :||Faculty of Health and Medical Sciences > School of Biosciences and Medicine > Department of Biochemical Sciences|
|Date :||30 March 2012|
|Identification Number :||10.1016/j.mpsur.2012.01.011|
|Additional Information :||NOTICE: this is the author’s version of a work that was accepted for publication in Surgery. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Surgery, 30(4), March 2012, DOI 10.1016/j.mpsur.2012.01.011.|
|Depositing User :||Symplectic Elements|
|Date Deposited :||09 May 2012 09:40|
|Last Modified :||23 Sep 2013 19:22|
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