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Spatiotemporal development of the bacterial community in a tubular longitudinal microbial fuel cell.

Kim, JR, Beecroft, NJ, Varcoe, JR, Dinsdale, RM, Guwy, AJ, Slade, RC, Thumser, A, Avignone-Rossa, C and Premier, GC (2011) Spatiotemporal development of the bacterial community in a tubular longitudinal microbial fuel cell. Appl Microbiol Biotechnol, 90 (3). 1179 - 1191. ISSN 0175-7598

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Abstract

The spatiotemporal development of a bacterial community in an exoelectrogenic biofilm was investigated in sucrose-fed longitudinal tubular microbial fuel cell reactors, consisting of two serially connected modules. The proportional changes in the microbial community composition were assessed by polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE) and DNA sequencing in order to relate them to the performance and stability of the bioelectrochemical system. The reproducibility of duplicated reactors, evaluated by cluster analysis and Jaccard's coefficient, shows 80-90% similarity in species composition. Biofilm development through fed-batch start-up and subsequent stable continuous operation results in a population shift from γ-Proteobacteria- and Bacteroidetes- to Firmicutes-dominated communities, with other diverse species present at much lower relative proportions. DGGE patterns were analysed by range-weighted richness (Rr) and Pareto-Lorenz evenness distribution curves to investigate the evolution of the bacterial community. The first modules shifted from dominance by species closely related to Bacteroides graminisolvens, Raoultella ornithinolytica and Klebsiella sp. BM21 at the start of continuous-mode operation to a community dominated by Paludibacter propionicigenes-, Lactococcus sp.-, Pantoea agglomerans- and Klebsiella oxytoca-related species with stable power generation (6.0 W/m(3)) at day 97. Operational strategies that consider the dynamics of the population will provide useful parameters for evaluating system performance in the practical application of microbial fuel cells.

Item Type: Article
Divisions : Faculty of Engineering and Physical Sciences > Chemistry
Authors :
AuthorsEmail
Kim, JRUNSPECIFIED
Beecroft, NJUNSPECIFIED
Varcoe, JRUNSPECIFIED
Dinsdale, RMUNSPECIFIED
Guwy, AJUNSPECIFIED
Slade, RCUNSPECIFIED
Thumser, AUNSPECIFIED
Avignone-Rossa, CUNSPECIFIED
Premier, GCUNSPECIFIED
Date : May 2011
Identification Number : 10.1007/s00253-011-3181-y
Uncontrolled Keywords : Bacteria, Bioelectric Energy Sources, Biofilms, DNA, Bacterial, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S, Sucrose
Additional Information : The original publication is available at http://www.springerlink.com
Depositing User : Symplectic Elements
Date Deposited : 13 Jun 2012 12:30
Last Modified : 26 Aug 2015 16:56
URI: http://epubs.surrey.ac.uk/id/eprint/375309

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