University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Spatiotemporal development of the bacterial community in a tubular longitudinal microbial fuel cell.

Kim, JR, Beecroft, NJ, Varcoe, JR, Dinsdale, RM, Guwy, AJ, Slade, RC, Thumser, A, Avignone-Rossa, C and Premier, GC (2011) Spatiotemporal development of the bacterial community in a tubular longitudinal microbial fuel cell. Appl Microbiol Biotechnol, 90 (3). 1179 - 1191. ISSN 0175-7598

[img]
Preview
PDF (Word to PDF conversion (via antiword) conversion from application/msword to application/pdf)
2011-0202-Bacterial_community_development_(AMB_Manuscript_ID_AMB-10-21602)97-2003.pdf
Available under License : See the attached licence file.

Download (601kB)
[img]
Preview
PDF (Word to PDF conversion (via antiword) conversion from application/msword to application/pdf)
2011-0202-Bacterial_community_development-Supplementary_Info_(AMB_Manuscript_ID_AMB-10-21602)97-2003.pdf
Available under License : See the attached licence file.

Download (147kB)
[img]
Preview
PDF (licence)
SRI_deposit_agreement.pdf

Download (33kB)

Abstract

The spatiotemporal development of a bacterial community in an exoelectrogenic biofilm was investigated in sucrose-fed longitudinal tubular microbial fuel cell reactors, consisting of two serially connected modules. The proportional changes in the microbial community composition were assessed by polymerase chain reaction-denaturing gradient gel electrophoresis (DGGE) and DNA sequencing in order to relate them to the performance and stability of the bioelectrochemical system. The reproducibility of duplicated reactors, evaluated by cluster analysis and Jaccard's coefficient, shows 80-90% similarity in species composition. Biofilm development through fed-batch start-up and subsequent stable continuous operation results in a population shift from γ-Proteobacteria- and Bacteroidetes- to Firmicutes-dominated communities, with other diverse species present at much lower relative proportions. DGGE patterns were analysed by range-weighted richness (Rr) and Pareto-Lorenz evenness distribution curves to investigate the evolution of the bacterial community. The first modules shifted from dominance by species closely related to Bacteroides graminisolvens, Raoultella ornithinolytica and Klebsiella sp. BM21 at the start of continuous-mode operation to a community dominated by Paludibacter propionicigenes-, Lactococcus sp.-, Pantoea agglomerans- and Klebsiella oxytoca-related species with stable power generation (6.0 W/m(3)) at day 97. Operational strategies that consider the dynamics of the population will provide useful parameters for evaluating system performance in the practical application of microbial fuel cells.

Item Type: Article
Additional Information: The original publication is available at http://www.springerlink.com
Uncontrolled Keywords: Bacteria, Bioelectric Energy Sources, Biofilms, DNA, Bacterial, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S, Sucrose
Divisions: Faculty of Engineering and Physical Sciences > Chemistry
Depositing User: Symplectic Elements
Date Deposited: 13 Jun 2012 12:30
Last Modified: 07 Oct 2014 10:19
URI: http://epubs.surrey.ac.uk/id/eprint/375309

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800