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Dam methyltransferase is required for stable lysogeny of the Shiga toxin (Stx2)-encoding bacteriophage 933W of enterohemorrhagic Escherichia coli O157:H7.

Murphy, KC, Ritchie, JM, Waldor, MK, Løbner-Olesen, A and Marinus, MG (2008) Dam methyltransferase is required for stable lysogeny of the Shiga toxin (Stx2)-encoding bacteriophage 933W of enterohemorrhagic Escherichia coli O157:H7. J Bacteriol, 190 (1). pp. 438-441.

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Abstract

Shiga toxin 2 (Stx2), one of the principal virulence factors of enterohemorrhagic Escherichia coli, is encoded by 933W, a lambda-like prophage. 933W prophage induction contributes to Stx2 production, and here, we provide evidence that Dam methyltransferase is essential for maintenance of 933W lysogeny. Our findings are consistent with the idea that the 933W prophage has a relatively low threshold for induction, which may promote Stx2 production during infection.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine > Department of Microbial and Cellular Sciences
Authors :
NameEmailORCID
Murphy, KCUNSPECIFIEDUNSPECIFIED
Ritchie, JMUNSPECIFIEDUNSPECIFIED
Waldor, MKUNSPECIFIEDUNSPECIFIED
Løbner-Olesen, AUNSPECIFIEDUNSPECIFIED
Marinus, MGUNSPECIFIEDUNSPECIFIED
Date : January 2008
Identification Number : 10.1128/JB.01373-07
Uncontrolled Keywords : Coliphages, Enterohemorrhagic Escherichia coli, Kanamycin, Lysogeny, Oligonucleotide Array Sequence Analysis, Polymerase Chain Reaction, Shiga Toxin 2, Site-Specific DNA-Methyltransferase (Adenine-Specific), Virulence
Depositing User : Symplectic Elements
Date Deposited : 21 Mar 2012 16:35
Last Modified : 31 Oct 2017 14:30
URI: http://epubs.surrey.ac.uk/id/eprint/326424

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