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Type 2 secretion promotes enterohemorrhagic Escherichia coli adherence and intestinal colonization.

Ho, TD, Davis, BM, Ritchie, JM and Waldor, MK (2008) Type 2 secretion promotes enterohemorrhagic Escherichia coli adherence and intestinal colonization. Infect Immun, 76 (5). pp. 1858-1865.

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Abstract

Enterohemorrhagic Escherichia coli (EHEC) is a noninvasive food-borne pathogen that colonizes the distal ileum and colon. Proteins encoded in the EHEC locus of enterocyte effacement (LEE) pathogenicity island are known to contribute to this pathogen's adherence to epithelial cells and intestinal colonization. The role of non-LEE-encoded proteins in these processes is not as clear. We found that the Z2053 gene (designated adfO here), a gene located in a cryptic EHEC prophage, exhibits similarity to adherence and/or colonization factor genes found in several other enteric pathogens. An EHEC adfO mutant exhibited marked reductions in adherence to HeLa cells and in the secretion of several proteins into the supernatant. YodA, one of these secreted proteins, was found to be a substrate of the EHEC pO157-encoded type 2 secretion system (T2SS). Both the T2SS and YodA proved to be essential for EHEC adherence to cultured HeLa cell monolayers. Using an infant rabbit model of infection, we found that the adfO mutation did not affect colonization but that the colonization of an etpC (T2SS) mutant was reduced approximately 5-fold. A strain deficient in YodA had a more severe colonization defect; however, this strain also exhibited a growth defect in vitro. Overall, our findings indicate that the pO157-encoded T2SS contributes to EHEC adherence and intestinal colonization and thus show that EHEC pathogenicity depends on type 2 secretion as well as type 3 secretion.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine > Department of Microbial and Cellular Sciences
Authors :
NameEmailORCID
Ho, TDUNSPECIFIEDUNSPECIFIED
Davis, BMUNSPECIFIEDUNSPECIFIED
Ritchie, JMUNSPECIFIEDUNSPECIFIED
Waldor, MKUNSPECIFIEDUNSPECIFIED
Date : May 2008
Identification Number : 10.1128/IAI.01688-07
Uncontrolled Keywords : Animals, Bacterial Adhesion, Bacterial Outer Membrane Proteins, Enterohemorrhagic Escherichia coli, Escherichia coli Infections, Escherichia coli Proteins, Gene Deletion, HeLa Cells, Humans, Intestines, Rabbits, Virulence, Virulence Factors
Depositing User : Symplectic Elements
Date Deposited : 21 Mar 2012 16:45
Last Modified : 31 Oct 2017 14:30
URI: http://epubs.surrey.ac.uk/id/eprint/326422

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