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Transient shielding of intimin and the type III secretion system of enterohemorrhagic and enteropathogenic Escherichia coli by a group 4 capsule.

Shifrin, Y, Peleg, A, Ilan, O, Nadler, C, Kobi, S, Baruch, K, Yerushalmi, G, Berdichevsky, T, Altuvia, S, Elgrably-Weiss, M , Abe, C, Knutton, S, Sasakawa, C, Ritchie, JM, Waldor, MK and Rosenshine, I (2008) Transient shielding of intimin and the type III secretion system of enterohemorrhagic and enteropathogenic Escherichia coli by a group 4 capsule. J Bacteriol, 190 (14). pp. 5063-5074.

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Abstract

Enterohemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC, respectively) strains represent a major global health problem. Their virulence is mediated by the concerted activity of an array of virulence factors including toxins, a type III protein secretion system (TTSS), pili, and others. We previously showed that EPEC O127 forms a group 4 capsule (G4C), and in this report we show that EHEC O157 also produces a G4C, whose assembly is dependent on the etp, etk, and wzy genes. We further show that at early time points postinfection, these G4Cs appear to mask surface structures including intimin and the TTSS. This masking inhibited the attachment of EPEC and EHEC to tissue-cultured epithelial cells, diminished their capacity to induce the formation of actin pedestals, and attenuated TTSS-mediated protein translocation into host cells. Importantly, we found that Ler, a positive regulator of intimin and TTSS genes, represses the expression of the capsule-related genes, including etp and etk. Thus, the expression of TTSS and G4C is conversely regulated and capsule production is diminished upon TTSS expression. Indeed, at later time points postinfection, the diminishing capsule no longer interferes with the activities of intimin and the TTSS. Notably, by using the rabbit infant model, we found that the EHEC G4C is required for efficient colonization of the rabbit large intestine. Taken together, our results suggest that temporal expression of the capsule, which is coordinated with that of the TTSS, is required for optimal EHEC colonization of the host intestine.

Item Type: Article
Divisions : Faculty of Health and Medical Sciences > School of Biosciences and Medicine > Department of Microbial and Cellular Sciences
Authors :
NameEmailORCID
Shifrin, YUNSPECIFIEDUNSPECIFIED
Peleg, AUNSPECIFIEDUNSPECIFIED
Ilan, OUNSPECIFIEDUNSPECIFIED
Nadler, CUNSPECIFIEDUNSPECIFIED
Kobi, SUNSPECIFIEDUNSPECIFIED
Baruch, KUNSPECIFIEDUNSPECIFIED
Yerushalmi, GUNSPECIFIEDUNSPECIFIED
Berdichevsky, TUNSPECIFIEDUNSPECIFIED
Altuvia, SUNSPECIFIEDUNSPECIFIED
Elgrably-Weiss, MUNSPECIFIEDUNSPECIFIED
Abe, CUNSPECIFIEDUNSPECIFIED
Knutton, SUNSPECIFIEDUNSPECIFIED
Sasakawa, CUNSPECIFIEDUNSPECIFIED
Ritchie, JMUNSPECIFIEDUNSPECIFIED
Waldor, MKUNSPECIFIEDUNSPECIFIED
Rosenshine, IUNSPECIFIEDUNSPECIFIED
Date : July 2008
Identification Number : 10.1128/JB.00440-08
Uncontrolled Keywords : Adhesins, Bacterial, Animals, Bacterial Adhesion, Bacterial Capsules, Cell Line, Enteropathogenic Escherichia coli, Epithelial Cells, Erythrocytes, Escherichia coli Infections, Escherichia coli O157, Escherichia coli Proteins, Gene Deletion, Gene Expression Profiling, Gene Expression Regulation, Bacterial, Glycosyltransferases, Humans, Intestine, Large, Membrane Glycoproteins, Membrane Proteins, Membrane Transport Proteins, Microscopy, Electron, Transmission, Mutagenesis, Insertional, Protein-Tyrosine Kinases, Rabbits, Trans-Activators, Virulence Factors
Depositing User : Symplectic Elements
Date Deposited : 21 Mar 2012 16:50
Last Modified : 31 Oct 2017 14:30
URI: http://epubs.surrey.ac.uk/id/eprint/326421

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