RNA-seq-based monitoring of infection-linked changes in vibrio cholerae gene expression

Mandlik, A, Livny, J, Robins, WP, Ritchie, JM, Mekalanos, JJ and Waldor, MK (2011) RNA-seq-based monitoring of infection-linked changes in vibrio cholerae gene expression Cell Host and Microbe, 10 (2). 165 - 174. ISSN 1931-3128

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Abstract

Pathogens adapt to the host environment by altering their patterns of geneexpression. Microarray-based and genetic techniques used to characterize bacterial geneexpression during infection are limited in their ability to comprehensively and simultaneously monitor genome-wide transcription. We used massively parallel cDNA sequencing (RNA-seq) techniques to quantitatively catalog the transcriptome of the cholera pathogen, Vibriocholerae, derived from two animal models of infection. Transcripts elevated in infected rabbits and mice relative to laboratory media derive from the major known V. cholerae virulence factors and also from genes and small RNAs not previously linked to virulence. The RNA-seq data was coupled with metabolite analysis of cecal fluid from infected rabbits to yield insights into the host environment encountered by the pathogen and the mechanisms controlling pathogen geneexpression. RNA-seq-based transcriptome analysis of pathogens during infection produces a robust, sensitive, and accessible data set for evaluation of regulatory responses driving pathogenesis.

Item Type:	Article
Additional Information:	NOTICE: this is the author’s version of a work that was accepted for publication in Cell Host & Microbe. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Cell Host & Microbe, 10(2), August 2011, DOI 10.1016/j.chom.2011.07.007.
Divisions:	Faculty of Health and Medical Sciences > Microbial and Cellular Sciences
Depositing User:	Symplectic Elements
Date Deposited:	31 May 2012 13:27
Last Modified:	09 Jun 2014 13:42
URI:	http://epubs.surrey.ac.uk/id/eprint/326413

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