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An Escherichia coli O157-specific engineered pyocin prevents and ameliorates infection by E. coli O157:H7 in an animal model of diarrheal disease.

Ritchie, JM, Greenwich, JL, Davis, BM, Bronson, RT, Gebhart, D, Williams, SR, Martin, D, Scholl, D and Waldor, MK (2011) An Escherichia coli O157-specific engineered pyocin prevents and ameliorates infection by E. coli O157:H7 in an animal model of diarrheal disease. Antimicrob Agents Chemother, 55 (12). pp. 5469-5474.

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Abstract

AvR2-V10.3 is an engineered R-type pyocin that specifically kills Escherichia coli O157, an enteric pathogen that is a major cause of food-borne diarrheal disease. New therapeutics to counteract E. coli O157 are needed, as currently available antibiotics can exacerbate the consequences of infection. We show here that orogastric administration of AvR2-V10.3 can prevent or ameliorate E. coli O157:H7-induced diarrhea and intestinal inflammation in an infant rabbit model of infection when the compound is administered either in a postexposure prophylactic regimen or after the onset of symptoms. Notably, administration of AvR2-V10.3 also reduces bacterial carriage and fecal shedding of this pathogen. Our findings support the further development of pathogen-specific R-type pyocins as a way to treat enteric infections.

Item Type: Article
Authors :
AuthorsEmailORCID
Ritchie, JMUNSPECIFIEDUNSPECIFIED
Greenwich, JLUNSPECIFIEDUNSPECIFIED
Davis, BMUNSPECIFIEDUNSPECIFIED
Bronson, RTUNSPECIFIEDUNSPECIFIED
Gebhart, DUNSPECIFIEDUNSPECIFIED
Williams, SRUNSPECIFIEDUNSPECIFIED
Martin, DUNSPECIFIEDUNSPECIFIED
Scholl, DUNSPECIFIEDUNSPECIFIED
Waldor, MKUNSPECIFIEDUNSPECIFIED
Date : December 2011
Identification Number : https://doi.org/10.1128/AAC.05031-11
Uncontrolled Keywords : Animals, Animals, Newborn, Anti-Bacterial Agents, Bacterial Load, Diarrhea, Disease Models, Animal, Escherichia coli Infections, Escherichia coli O157, Feces, Genetic Engineering, Humans, Pyocins, Rabbits, Treatment Outcome
Depositing User : Symplectic Elements
Date Deposited : 28 Mar 2017 14:41
Last Modified : 28 Mar 2017 14:41
URI: http://epubs.surrey.ac.uk/id/eprint/326412

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