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Polyaniline nanofibres as templates for the covalent immobilisation of biomolecules

Crean (nee Lynam), C, Lahiff, E, Gilmartin, N, Diamond, D and O'Kennedy, R (2011) Polyaniline nanofibres as templates for the covalent immobilisation of biomolecules SYNTHETIC METALS, 161 (3-4). 285 - 292. ISSN 0379-6779

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The attachment of antibodies onto polyaniline nanofibres using covalent chemistry was investigated for the first time. Polyaniline nanofibres were functionalised post-polymerisation to attach either amide or carboxylic acid side-groups. These templates could then be further modified to attach antibodies, specifically in this instance mouse immunoglobulin G (IgG). The resultant conjugates were characterised using a variety of techniques including infrared. UV-vis and Raman spectroscopy. Conjugates were then used to detect secondary antibodies (anti-IgG). Results from enzyme-linked immunoassay studies indicate successful binding of the antibody to the polyaniline nanofibres. Carboxyl functionalised polyaniline nanofibres are shown in particular to decrease non-specific binding in the immunoassay. Direct electrical communication between polyaniline nanofibres covalently linked to peroxidase-labelled antibodies was observed during cyclic voltammetry, which demonstrates their potential for further development as nano-dimensional immunosensors. (C) 2010 Elsevier B.V. All rights reserved.

Item Type: Article
Uncontrolled Keywords: Science & Technology, Technology, Physical Sciences, Materials Science, Multidisciplinary, Physics, Condensed Matter, Polymer Science, Materials Science, Physics, Polyaniline nanofibres, Conducting polymer functionalisation, Antibody conjugation, Immunoassay, CONDUCTING POLYMERS, SENSORS, FILMS
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Divisions: Faculty of Engineering and Physical Sciences > Chemistry
Depositing User: Symplectic Elements
Date Deposited: 23 Jun 2011 11:28
Last Modified: 08 Nov 2013 12:08

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