Strong polyadenylation and weak pausing combine to cause efficient termination of transcription in the human Gγ-globin gene

Plant, KE, Dye, MJ, Lafaille, C and Proudfoot, NJ (2005) Strong polyadenylation and weak pausing combine to cause efficient termination of transcription in the human Gγ-globin gene Molecular and Cellular Biology, 25 (8). pp. 3276-3285.

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Abstract

The human γ-globin genes form part of a 5-kb tandem duplication within the β-globin gene cluster on chromosome 11. Despite a high degree of identity between the two genes, we show that while the upstream Gγ-globin gene terminates transcription efficiently, termination in the Aγ gene is inefficient. This is primarily due to the different strengths of the poly(A) signals of the two genes; Gγ-globin has a functionally stronger poly(A) signal than the Aγ gene. The probable cause of this difference in poly(A) efficiency characteristics lies with a number of base changes which reduce the G/U content of the GU/U-rich region of the Aγ poly(A) signal relative to that of Gγ. The 3′ flanking regions of the two γ-globin genes have similar abilities to promote transcription termination. We found no evidence to suggest a cotranscriptional cleavage event, such as that seen in the human β-globin gene, occurs in either γ-globin 3′ flank. Instead we find evidence that the 3′ flank of the Gγ-globin gene contains multiple weak pause elements which, combined with the strong poly(A) signal the gene possesses, are likely to cause gradual termination across the 3′ flank.

Item Type:	Article
Divisions :	Faculty of Health and Medical Sciences > School of Biosciences and Medicine > Department of Biochemical Sciences
Authors :	Authors Email ORCID Plant, KE UNSPECIFIED UNSPECIFIED Dye, MJ UNSPECIFIED UNSPECIFIED Lafaille, C UNSPECIFIED UNSPECIFIED Proudfoot, NJ UNSPECIFIED UNSPECIFIED
Date :	2005
Identification Number :	https://doi.org/10.1128/​MCB.25.8.3276-3285.2005
Additional Information :	Copyright © American Society for Microbiology
Depositing User :	Symplectic Elements
Date Deposited :	09 May 2012 09:49
Last Modified :	23 Sep 2013 19:17
URI:	http://epubs.surrey.ac.uk/id/eprint/285508

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