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EXPERIMENTAL SHORT-TERM PARTIAL OBSTRUCTION OF THE FETAL SHEEP BLADDER OUTFLOW: II. COMPLIANCE AND CONTRACTILITY ASSOCIATED WITH URINARY FLOW IMPAIRMENT

Fry, CH, Farrugia, MK, Godley, ML, Woolf, AS, Peebles, DM and Cuckow, PM (2006) EXPERIMENTAL SHORT-TERM PARTIAL OBSTRUCTION OF THE FETAL SHEEP BLADDER OUTFLOW: II. COMPLIANCE AND CONTRACTILITY ASSOCIATED WITH URINARY FLOW IMPAIRMENT Journal of Paediatric Urology, 2 (4). 254 - 260.

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Abstract

Purpose Posterior urethral valves (PUV) is the commonest cause of congenital bladder outlet obstruction. Despite valve ablation in the neonatal period, up to 70% of patients develop renal failure by their teenage years, and progressive bladder dysfunction. This study forms part of a continuing project examining the relationship between severity and duration of obstruction and urinary tract dysfunction. Here is the assessed result of short-term (9-day) obstruction. Materials and methods Fourteen male fetal lambs at 75 days' gestation were assigned to one of three groups: urachal ligation, urachal ligation with partial urethral obstruction, sham-operated controls. Pregnancy proceeded for 9 days. At autopsy, filling cystometry was performed with the urinary tract in situ and the bladder harvested for nerve counts using PGP 9.5 immunohistochemistry, or in vitro measurement of contractile function. Results Obstruction was associated with an increase in bladder:fetal weight ratio. Compliance was variable in the obstructed bladders, but the calculated wall stress per unit strain was either similar or less than controls. Nerve-mediated or agonist-induced contraction magnitude in tissue from obstructed bladders and nerve counts did not differ from controls. Conclusions Nine days of outflow obstruction at mid-gestation generated a bladder of increased weight but without evidence of contractile failure. An increase in bladder compliance as a function of bladder growth was observed even at this stage, and represents one of the initial responses to outflow tract obstruction.

Item Type: Article
Additional Information: NOTICE: this is the author’s version of a work that was accepted for publication in Journal of Paediatric Urology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Journal of Paediatric Urology, 2(4), June 2006, DOI 10.1016/j.jpurol.2006.02.006.
Divisions: Faculty of Health and Medical Sciences > Biochemistry and Physiology
Depositing User: Symplectic Elements
Date Deposited: 09 May 2012 10:41
Last Modified: 23 Sep 2013 19:17
URI: http://epubs.surrey.ac.uk/id/eprint/285494

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