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Modulation of bladder myofibroblast activity: implications for bladder function

Sui, G-P, Wu, C, Roosen, A, Ikeda, Y, Kanai, AJ and Fry, CH (2008) Modulation of bladder myofibroblast activity: implications for bladder function AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, 295 (3). F688-F697.

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Abstract

Bladder suburothelial myofibroblasts may modulate both sensory responses from the bladder wall and spontaneous activity. This study aimed to characterize further these cells in their response to exogenous agents implicated in mediating the above activity. Detrusor strips, with or without mucosa, and isolated suburothelial myofibroblasts were prepared from guinea pig bladders. Isometric tension, intracellular Ca2+, and membrane current were recorded. Cell pairs were formed by pushing two cells together. Tension, intracellular Ca2+, and membrane potential were also recorded from bladder sheets using normal or spinal cord-transected (SCT) rats. Spontaneous contractions were greater in detrusor strips with an intact mucosa and were augmented by 10 μM UTP. ATP, UTP, or reduced extracellular pH elicited Ca2+ transients and inward currents (Erev −30 mV) in isolated cells. Capsaicin (5–30 μM) reduced membrane current (37 ± 12% of control) with minor effects on Ca2+ transients: sodium nitroprusside reduced membrane currents (40 ± 21% of control). Cell pair formation, without an increase in cell capacitance, augmented ATP and pH responses (180 ± 58% of control) and reduced the threshold to ATP and acidosis. Glivec (20–50 μM) reversibly blocked the augmentation and also reduced spontaneous activity in bladder sheets from SCT, but not normal, rats. Glivec also disrupted the spread of Ca2+ waves in SCT sheets, generating patterns similar to normal bladders. Suburothelial myofibroblasts respond to exogenous agents implicated in modulating bladder sensory responses; responses augmented by physical intercellular contact. The action of glivec and its selective suppression of spontaneous activity in SCT rats identifies a possible pathway to attenuate bladder overactivity.

Item Type: Article
Subjects : Biological Sciences
Divisions : Faculty of Health and Medical Sciences
Authors :
AuthorsEmailORCID
Sui, G-PUNSPECIFIEDUNSPECIFIED
Wu, CUNSPECIFIEDUNSPECIFIED
Roosen, AUNSPECIFIEDUNSPECIFIED
Ikeda, YUNSPECIFIEDUNSPECIFIED
Kanai, AJUNSPECIFIEDUNSPECIFIED
Fry, CHUNSPECIFIEDUNSPECIFIED
Date : 1 September 2008
Identification Number : 10.1152/ajprenal.00133.2008
Copyright Disclaimer : Copyright 2008 The American Physiological Society.
Uncontrolled Keywords : Science & Technology, Life Sciences & Biomedicine, Physiology, Urology & Nephrology, PHYSIOLOGY, UROLOGY & NEPHROLOGY, suburothelium, spontaneous activity, purinergic signaling, DETRUSOR SMOOTH-MUSCLE, PIG SUBUROTHELIAL MYOFIBROBLASTS, STEM-CELL FACTOR, GUINEA-PIG, URINARY-BLADDER, INTERSTITIAL-CELLS, C-KIT, VANILLOID RECEPTOR, EPITHELIAL-CELLS, TYROSINE KINASE
Related URLs :
Additional Information : Copyright 2008 The American Physiological Society.
Depositing User : Symplectic Elements
Date Deposited : 08 Mar 2016 15:56
Last Modified : 08 Mar 2016 15:56
URI: http://epubs.surrey.ac.uk/id/eprint/285481

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