Towards the immunoproteome of Neisseria meningitidis.
Mendum, TA, Newcombe, J, McNeilly, CL and McFadden, J (2009) Towards the immunoproteome of Neisseria meningitidis. PLoS One, 4 (6). e5940 - ?. ISSN 1932-6203
towards_immunoproteome_MENDUM_09.pdf - Published Version
Despite the introduction of conjugated polysaccharide vaccines for many of the Neisseria meningitidis serogroups, neisserial infections continue to cause septicaemia and meningitis across the world. This is in part due to the difficulties in developing a, cross-protective vaccine that is effective against all serogroups, including serogroup B meningococci. Although convalescent N. meningitidis patients develop a natural long-lasting cross-protective immunity, the antigens that mediate this response remain unknown. To help define the target of this protective immunity we identified the proteins recognized by IgG in sera from meningococcal patients by a combination of 2D protein gels, western blots and mass spectrometry. Although a number of outer membrane antigens were identified the majority of the antigens were cytoplasmic, with roles in cellular processes and metabolism. When recombinant proteins were expressed and used to raise sera in mice, none of the antigens elicited a positive SBA result, however flow cytometry did demonstrate that some, including the ribosomal protein, RplY were localised to the neisserial cell surface.
|Uncontrolled Keywords:||Animals, Antigens, Bacterial, Cytoplasm, Female, Humans, Immune System, Immunoglobulin G, Mass Spectrometry, Meningitis, Meningococcal, Mice, Neisseria meningitidis, Proteome, Proteomics, Recombinant Proteins, Sepsis|
|Divisions:||Faculty of Health and Medical Sciences > Microbial and Cellular Sciences|
|Depositing User:||Melanie Hughes|
|Date Deposited:||26 Oct 2010 10:12|
|Last Modified:||23 Sep 2013 18:39|
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