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Acidic pH shock induces the expressions of a wide range of stress-response genes

Kim, YJ, Moon, MH, Song, JY, Smith, CP, Hong, S-K and Chang, YK (2008) Acidic pH shock induces the expressions of a wide range of stress-response genes BMC GENOMICS, 9. ? - ?. ISSN 1471-2164

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Abstract

Background: Environmental signals usually enhance secondary metabolite production in Streptomycetes by initiating complex signal transduction system. It is known that different sigma factors respond to different types of stresses, respectively in Streptomyces strains, which have a number of unique signal transduction mechanisms depending on the types of environmental shock. In this study, we wanted to know how a pH shock would affect the expression of various sigma factors and shock-related proteins in S. coelicolor A3(2). Results: According to the results of transcriptional and proteomic analyses, the major number of sigma factor genes were upregulated by an acidic pH shock. Well-studied sigma factor genes of sigH (heat shock), sigR (oxidative stress), sigB (osmotic shock), and hrdD that play a major role in the secondary metabolism, were all strongly upregulated by the pH shock. A number of heat shock proteins including the DnaK family and chaperones such as GroEL2 were also observed to be upregulated by the pH shock, while their repressor of hspR was strongly downregulated. Oxidative stress-related proteins such as thioredoxin, catalase, superoxide dismutase, peroxidase, and osmotic shock-related protein such as vesicle synthases were also upregulated in overall. Conclusion: From these observations, an acidic pH shock was considered to be one of the strongest stresses to influence a wide range of sigma factors and shock-related proteins including general stress response proteins. The upregulation of the sigma factors and shock proteins already found to be related to actinorhodin biosynthesis was considered to have contributed to enhanced actinorhodin productivity by mediating the pH shock signal to regulators or biosynthesis genes for actinorhodin production

Item Type: Article
Additional Information: © 2008 Kim et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Uncontrolled Keywords: Science & Technology, Life Sciences & Biomedicine, Biotechnology & Applied Microbiology, Genetics & Heredity, STREPTOMYCES-COELICOLOR A3(2), CONTAINING SUPEROXIDE-DISMUTASE, POLYKETIDE SYNTHASE COMPONENTS, POLYMERASE SIGMA-FACTOR, PYRAN RING FORMATION, ACTINORHODIN BIOSYNTHESIS, TRANSCRIPTIONAL REGULATOR, PROPER DIFFERENTIATION, KASUGAMYCIN PRODUCTION, IDENTIFICATION
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Divisions: Faculty of Health and Medical Sciences > Microbial and Cellular Sciences
Depositing User: Melanie Hughes
Date Deposited: 24 Feb 2012 14:51
Last Modified: 23 Sep 2013 19:14
URI: http://epubs.surrey.ac.uk/id/eprint/225052

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