University of Surrey

Test tubes in the lab Research in the ATI Dance Research

Affinity purification of ribosomes to access the translatome.

Halbeisen, RE, Scherrer, T and Gerber, AP (2009) Affinity purification of ribosomes to access the translatome. Methods, 48 (3). pp. 306-310.

[img] PDF
Halbeisen et al. 2009_Methods.pdf
Restricted to Repository staff only
Available under License : See the attached licence file.

Download (316kB)
[img] PDF (licence)
SRI_deposit_agreement.pdf
Restricted to Repository staff only

Download (33kB)

Abstract

We describe ribosome affinity purification (RAP), a method that allows rapid purification of ribosomes and associated messages from the yeast Saccharomyces cerevisiae. The method relies on the expression of protein A tagged versions of the ribosomal protein Rpl16, which is used to efficiently recover endogenously formed ribosomes and polysomes from cellular extracts with IgG-coupled spherical microbeads. This approach can be applied to profile reactions of the translatome, which refers to all messages associated with ribosomes, with those of the transcriptome using DNA microarrays. In addition, ribosomal proteins, their modifications, and/or other associated proteins can be mapped with mass spectrometry. Finally, application of this method in other organisms provides a valuable tool to decipher cell-type specific gene expression patterns.

Item Type: Article
Authors :
AuthorsEmailORCID
Halbeisen, REUNSPECIFIEDUNSPECIFIED
Scherrer, TUNSPECIFIEDUNSPECIFIED
Gerber, APUNSPECIFIEDUNSPECIFIED
Date : July 2009
Identification Number : https://doi.org/10.1016/j.ymeth.2009.04.003
Uncontrolled Keywords : Cell Fractionation, Chromatography, Affinity, Protein Array Analysis, RNA-Binding Proteins, Ribosomal Proteins, Ribosomes, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 28 Mar 2017 14:39
Last Modified : 28 Mar 2017 14:39
URI: http://epubs.surrey.ac.uk/id/eprint/211754

Actions (login required)

View Item View Item

Downloads

Downloads per month over past year


Information about this web site

© The University of Surrey, Guildford, Surrey, GU2 7XH, United Kingdom.
+44 (0)1483 300800