Pumilio binds para mRNA and requires Nanos and Brat to regulate sodium current in drosophila motoneurons
Muraro, NI, Weston, AJ, Gerber, AP, Luschnig, S, Moffat, KG and Baines, RA (2008) Pumilio binds para mRNA and requires Nanos and Brat to regulate sodium current in drosophila motoneurons Journal of Neuroscience, 28 (9). 2099 - 2109. ISSN 0270-6474
Muraro et al. 2008 JNS.pdf - Published Version
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Homeostatic regulation of ionic currents is of paramount importance during periods of synaptic growth or remodeling. Our previous work has identified the translational repressor Pumilio (Pum) as a regulator of sodium current (I Na) and excitability in Drosophila motoneurons. In this current study, we show that Pum is able to bind directly the mRNA encoding the Drosophila voltage-gated sodium channel paralytic (para). We identify a putative binding site for Pum in the 3′ end of the para open reading frame (ORF). Characterization of the mechanism of action of Pum, using whole-cell patch clamp and real-time reverse transcription-PCR, reveals that the full-length protein is required for translational repression of para mRNA. Additionally, the cofactor Nanos is essential for Pum-dependent para repression, whereas the requirement for Brain Tumor (Brat) is cell type specific. Thus, Pum-dependent regulation of I Na in motoneurons requires both Nanos and Brat, whereas regulation in other neuronal types seemingly requires only Nanos but not Brat. We also show that Pum is able to reduce the level of nanos mRNA and as such identify a potential negative-feedback mechanism to protect neurons from overactivity of Pum. Finally, we show coupling between I Na (para) and I K (Shal) such that Pum-mediated change in para results in a compensatory change in Shal. The identification of para as a direct target of Pum represents the first ion channel to be translationally regulated by this repressor and the location of the binding motif is the first example in an ORF rather than in the canonical 3′-untranslated region of target transcripts.
|Additional Information:||Copyright 2008 Society for Neuroscience|
|Divisions:||Faculty of Health and Medical Sciences > Microbial and Cellular Sciences|
|Depositing User:||Symplectic Elements|
|Date Deposited:||18 Apr 2012 11:19|
|Last Modified:||23 Sep 2013 19:13|
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