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Genome-wide identification of mRNAs associated with the translational regulator PUMILIO in Drosophila melanogaster.

Gerber, AP, Luschnig, S, Krasnow, MA, Brown, PO and Herschlag, D (2006) Genome-wide identification of mRNAs associated with the translational regulator PUMILIO in Drosophila melanogaster. Proc Natl Acad Sci U S A, 103 (12). pp. 4487-4492.

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Abstract

Genome-wide identification of RNAs associated with RNA-binding proteins is crucial for deciphering posttranscriptional regulatory systems. PUMILIO is a member of the evolutionary conserved Puf-family of RNA-binding proteins that repress gene expression posttranscriptionally. We generated transgenic flies expressing affinity-tagged PUMILIO under the control of an ovary-specific promoter, and we purified PUMILIO from whole adult flies and embryos and analyzed associated mRNAs by using DNA microarrays. Distinct sets comprising hundreds of mRNAs were associated with PUMILIO at the two developmental stages. Many of these mRNAs encode functionally related proteins, supporting a model for coordinated regulation of posttranscriptional modules by specific RNA-binding proteins. We identified a characteristic sequence motif in the 3'-untranslated regions of mRNAs associated with PUMILIO, and the sufficiency of this motif for interaction with PUMILIO was confirmed by RNA pull-down experiments with biotinylated synthetic RNAs. The RNA motif strikingly resembles the one previously identified for Puf3p, one of five Saccharomyces cerevisiae Puf proteins; however, proteins encoded by the associated mRNAs in yeast and Drosophila do not appear to be related. The results suggest extensive posttranscriptional regulation by PUMILIO and uncover evolutionary features of this conserved family of RNA-binding proteins.

Item Type: Article
Authors :
NameEmailORCID
Gerber, APUNSPECIFIEDUNSPECIFIED
Luschnig, SUNSPECIFIEDUNSPECIFIED
Krasnow, MAUNSPECIFIEDUNSPECIFIED
Brown, POUNSPECIFIEDUNSPECIFIED
Herschlag, DUNSPECIFIEDUNSPECIFIED
Date : 21 March 2006
Identification Number : 10.1073/pnas.0509260103
Uncontrolled Keywords : 3' Untranslated Regions, Animals, Animals, Genetically Modified, Base Sequence, Drosophila Proteins, Drosophila melanogaster, Evolution, Molecular, Gene Expression Regulation, Genome, Insect, Molecular Sequence Data, Oligonucleotide Array Sequence Analysis, Protein Biosynthesis, RNA, Messenger, RNA-Binding Proteins, Saccharomyces cerevisiae
Related URLs :
Depositing User : Symplectic Elements
Date Deposited : 28 Mar 2017 14:39
Last Modified : 31 Oct 2017 14:26
URI: http://epubs.surrey.ac.uk/id/eprint/211745

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