A screen for RNA-binding proteins in yeast indicates dual functions for many enzymes.
Scherrer, T, Mittal, N, Janga, SC and Gerber, AP (2010) A screen for RNA-binding proteins in yeast indicates dual functions for many enzymes. PLoS One, 5 (11). e15499 - ?. ISSN 1932-6203
Scherrer et al. PLoS One 2010.pdf
Available under License : See the attached licence file.
Hundreds of RNA-binding proteins (RBPs) control diverse aspects of post-transcriptional gene regulation. To identify novel and unconventional RBPs, we probed high-density protein microarrays with fluorescently labeled RNA and selected 200 proteins that reproducibly interacted with different types of RNA from budding yeast Saccharomyces cerevisiae. Surprisingly, more than half of these proteins represent previously known enzymes, many of them acting in metabolism, providing opportunities to directly connect intermediary metabolism with posttranscriptional gene regulation. We mapped the RNA targets for 13 proteins identified in this screen and found that they were associated with distinct groups of mRNAs, some of them coding for functionally related proteins. We also found that overexpression of the enzyme Map1 negatively affects the expression of experimentally defined mRNA targets. Our results suggest that many proteins may associate with mRNAs and possibly control their fates, providing dense connections between different layers of cellular regulation.
|Divisions :||Faculty of Health and Medical Sciences > Microbial and Cellular Sciences|
|Identification Number :||10.1371/journal.pone.0015499|
|Uncontrolled Keywords :||Aminopeptidases, Enzymes, Gene Expression Profiling, Oligonucleotide Array Sequence Analysis, Protein Array Analysis, Protein Binding, Proteome, Proteomics, RNA, Fungal, RNA, Messenger, RNA-Binding Proteins, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins|
|Additional Information :||Copyright 2010 Scherrer et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
|Depositing User :||Symplectic Elements|
|Date Deposited :||15 Mar 2012 14:05|
|Last Modified :||23 Sep 2013 19:13|
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