Quantification and confocal imaging of protein specific molecularly imprinted polymers
Hawkins, DM, Trache, A, Ellis, EA, Stevenson, D, Holzenburg, A, Meininger, GA and Reddy, SM (2006) Quantification and confocal imaging of protein specific molecularly imprinted polymers BIOMACROMOLECULES, 7 (9). 2560 - 2564. ISSN 1525-7797
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We have employed FITC-albumin as the protein template molecule in an aqueous phase molecular imprinted polymer (HydroMIP) strategy. For the first time, the use of a fluorescently labelled template is reported, with subsequent characterisation of the smart material to show that the HydroMIP possess a significant molecular memory in comparison to that of the nonimprinted control polymer (HydroNIP). The imaging of the FITC-albumin imprinted HydroMIP using confocal microscopy is described, with the in situ removal of imprinted protein displayed in terms of observed changes in the fluorescence of the imprinted polymer, both before and after template elution (using a 10% SDS/10% AcOH (w/v) solution). We also report the imaging of a bovine haemoglobin (BHb) imprinted HydroMIP using two-photon confocal microscopy, and describe the effects of template elution upon protein autofluorescence. The findings further contribute to the understanding of aqueous phase molecular imprinting protocols, and document the use of fluorescence as a useful tool in template labelling/detection and novel imaging strategies.
|Additional Information:||This document is the Accepted Manuscript version of a Published Work that appeared in final form in BIOMACROMOLECULES, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see http://dx.doi.org/10.1021/bm060494d|
|Uncontrolled Keywords:||Science & Technology, Life Sciences & Biomedicine, Physical Sciences, Biochemistry & Molecular Biology, Chemistry, Organic, Polymer Science, Chemistry, FLUORESCENCE, RESOLUTION, HYDROGELS|
|Divisions:||Faculty of Health and Medical Sciences > Biochemistry and Physiology|
|Depositing User:||Symplectic Elements|
|Date Deposited:||09 Aug 2012 09:47|
|Last Modified:||09 Jun 2014 13:29|
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