Paracetamol inhibits nitric oxide synthesis in murine spinal cord slices
Godfrey, L, Bailey, I, Toms, NJ, Clarke, GD, Kitchen, I and Hourani, SMO (2007) Paracetamol inhibits nitric oxide synthesis in murine spinal cord slices EUR J PHARMACOL, 562 (1-2). 68 - 71. ISSN 0014-2999
Godfrey et al 2nd revision Fig 1.ppt
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Paracetamol is an effective analgesic but its mechanism of action is unclear. We investigated the effect of paracetamol and the analgesic adjuvant caffeine on the activity of NO synthase in mouse spinal cord and cerebellar slices in vitro, by measuring the conversion of [3H]arginine to [3H]citrulline. Paracetamol (100 μM) had no effect on NO synthase activity in cerebellum, but in the spinal cord both paracetamol (100 μM) and caffeine (30 μM) attenuated glutamate (5 mM)-induced [3H]citrulline production and in combination they abolished it. In conclusion paracetamol inhibits spinal cord NO synthesis and this may be related to its analgesic effects.
|Additional Information:||Copyright © 2007 Elsevier B.V. All rights reserved. NOTICE: this is the author’s version of a work that was accepted for publication in European Journal of Pharmacology. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in European Journal of Pharmacology, 562(1-2), May 2007, DOI: 10.1016/j.ejphar.2007.01.075|
|Uncontrolled Keywords:||paracetamol, caffeine, NO, spinal cord, mice, NO SYNTHASE INHIBITORS, ANTINOCICEPTIVE ACTION, FORMALIN TEST, L-NAME, ACETAMINOPHEN, CAFFEINE, NMDA, RAT, CYCLOOXYGENASE, HYPERALGESIA|
|Divisions:||Faculty of Health and Medical Sciences > Biochemistry and Physiology|
|Depositing User:||Symplectic Elements|
|Date Deposited:||07 Mar 2012 16:23|
|Last Modified:||23 Sep 2013 19:06|
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