Carbon flux distribution in antibiotic-producing chemostat cultures of Streptomyces lividans.
Avignone Rossa, C, White, J, Kuiper, A, Postma, PW, Bibb, M and Teixeira de Mattos, MJ (2002) Carbon flux distribution in antibiotic-producing chemostat cultures of Streptomyces lividans. Metab Eng, 4 (2). 138 - 150. ISSN 1096-7176
The carbon metabolism of derivatives of Streptomyces lividans growing under phosphate limitation in chemostat cultures and producing the antibiotics actinorhodin and undecylprodigiosin was investigated. By applying metabolic flux analysis to a stoichiometric model, the relationship between antibiotic production, biomass accumulation, and carbon flux through the major carbon metabolic pathways (the Embden Meyerhoff Parnas and pentose-phosphate pathways) was analyzed. Distribution of carbon flux through the catabolic pathways was shown to be dependent on growth rate, as well as on the carbon and energy source (glucose or gluconate) used. Increasing growth rates promoted an increase in the flux of carbon through glycolysis and the pentose-phosphate pathway. The synthesis of both actinorhodin and undecylprodigiosin was found to be inversely related to flux through the pentose-phosphate pathway.
|Additional Information:||NOTICE: This is the author’s version of a work that was accepted for publication in Metabolic Engineering. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Metabolic Engineering, 4(2), April 2002, DOI: 10.1006/mben.2001.0217|
|Uncontrolled Keywords:||Anthraquinones, Anti-Bacterial Agents, Biomedical Engineering, Bioreactors, Carbon, Kinetics, Models, Biological, Pentose Phosphate Pathway, Prodigiosin, Streptomyces|
|Divisions:||Faculty of Health and Medical Sciences > Microbial and Cellular Sciences|
|Depositing User:||Symplectic Elements|
|Date Deposited:||20 Apr 2012 08:46|
|Last Modified:||23 Sep 2013 19:06|
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